Content:20µgOverview
Stem
MACS™Klf4mRNAencodesthetranscriptionfactorKrüppel-likefactor4(Klf4)fortransienttransfection.
Applications
- Modulationofpluripotency
- Reprogramming
Details
Backgroundinformation
Krüppel-likefactor4(Klf4)isazincfingertranscriptionfactorthatplaysanimportantroleinmaintainingpluripotentstemcellsandpreventingtheirdifferentiation.Klf4regulatescelllineagedifferentiationduringdevelopmentandmaintainsepithelialhomeostasisintheintestine.ExogenousexpressionofKlf4incombinationwithothertranscriptionfactorshasbeenusedtogenerateinducedpluripotentstemcells.
Detailedprocedure
Forsatisfactorytransfectionresults,useatransfectionprotocoloptimizedforyourspecificcelltype.StemMACS™eGFPmRNAorStemMACS™NucleareGFPmRNAalloweasyevaluationoftransfectionefficiencyandarerecommendedaspositivecontrols.
Qualitydescription
StemMACS™mRNAsarehighlypure,invitro-transcribedmRNAsthathavebeencarefullyoptimizedtoensurehighlevelexpressionaftertransfection.PolyadenylationandenzymaticcapmimicafullyprocessedmaturemRNAandensureefficienttranslation.Incorporationofthemodifiednucleotidespseudouridineand5-methylcytidinereducestheinnateantiviralresponsetosingle-strandedmRNA.Robustexpressionoftheencodedfactoraftertransfectionhasbeenverifiedbyimmunofluorescenceorflowcytometry.
StemMACS™mRNAsarelyophilizedfroma0.2µMfilteredsolutionforextendedshelf-life.