ThekitconsistsofAnti-Biotin
MACSiBead™Particlesandbiotinylatedantibodiesagainstnon-humanprimateCD3,andhumanCD2andCD28.Anti-BiotinMACSiBead™Particlesloadedwithbiotinylatedantibodiesareusedtomimicantigen-presentingcellsandactivaterestingTcellsfromPBMCsaswellaspurifiedTcells.Tcellexpansionisachievedbyculturingandreactivationatday14ofculture.
ActivatedTcellscanbeusedformanydownstreamapplications,suchascytokineanalysisorimmunoprecipitation,andcellscanalsobetransfectedwithhighefficiency.