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NEB/CLIP-Surface™ 647/50 nmol/S9234S
  • NEB/CLIP-Surface™ 647/50 nmol/S9234S

NEB/CLIP-Surface™ 647/50 nmol/S9234S

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货号: S9234S
品牌: NEB
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    • This package includes 50 nmol of CLIP-Surface 647 substrate, sufficient to make 10 ml of a 5 µM CLIP-tag fusion protein labeling solution.The CLIP-tag protein labeling system enables the specific, covalent attachment of virtually any molecule to a protein of interest. CLIP-tag is a protein tag based on human O6-alkylguanine-DNA-alkyltransferase (hAGT). CLIP-tag substrates are derivatives of benzylcytosine (BC). In the labeling reaction, the substituted benzyl group of the substrate is covalently attached to the reactive cysteine of CLIP-tag forming a stable thioether bond. Although CLIP-tag is based on the same protein as SNAP-tag®, the benzylcytosine substrates form a separate class of substrates, different from the benzylguanine substrates recognized by SNAP-tag. CLIP-tag and SNAP-tag can be used for orthogonal and complementary labeling of two proteins simultaneously in the same cells.There are two steps to using this system: subcloning and expression of the protein of interest as a CLIP-tag fusion, and labeling of the fusion with the CLIP-tag substrate of choice. Expression of CLIP-tag fusion proteins is described in the documentation supplied with CLIP-tag plasmids. The labeling of the fusion proteins on the cell surface with the CLIP-tag substrate is described in this document.
      Figure 1:Excitation (dotted line) and emission (full line) spectra of CLIP-Surface 647 coupled to CLIP-tag in buffer at pH 7.5Figure 1: Excitation (dotted line) and emission (full line) spectra of CLIP-Surface 647 coupled to CLIP-tag in buffer at pH 7.5
      9147a_thumb
      Figure 2. Structure of CLIP-Surface 647 (MW 877.0 g/mol)
      This product is related to the following categories:
      CLIP-tag™ Substrates Products,
      Protein Labeling Products
      This product can be used in the following applications:
      CLIP Surface,
      In vivo Imaging,
      Pulse Chase,
      Receptor Internalization,
      Protein Localization,
      Protein Labeling Snap Clip
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