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NEB/SNAP-Cell® 505-Star/50 nmol/S9103S
  • NEB/SNAP-Cell® 505-Star/50 nmol/S9103S

NEB/SNAP-Cell® 505-Star/50 nmol/S9103S

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货号: S9103S
品牌: NEB
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    • This package contains 50nmol of SNAP-Cell 505-Starsubstrate, sufficient to make 10 ml of a 5 µM SNAP-tag fusion protein labeling solution.The SNAP-tag protein labeling system enables the specific, covalent attachment of virtually any molecule to a protein of interest. The SNAP-tag is a protein based on human O6-alkylguanine-DNA alkyltransferase (hAGT). SNAP-tag substrates arefluorophores, biotin or beads conjugated to guanine or chloropyrimidine leaving groups via a benzyl linker. In the labeling reaction, the substituted benzyl group of the substrate is covalently attached to the SNAP-tag.There are two steps to using this system: subcloning and expression of the protein of interest as a SNAP-tag fusion, and labeling of the fusion with the SNAP-tag substrate of choice. Expression of SNAP-tag fusion proteins is described in the documentation supplied with SNAP-tag plasmids. The labeling of the fusion proteins with the SNAP-tag substrate is described below.
      Figure 1:Live U-2 OS cells stably transfected with pSNAPf-H2BFigure 1: Live U-2 OS cells stably transfected with pSNAPf-H2B
      Cells were labeled with SNAP-Cell 505-Star(green) for 15 minutes at 37°C, 5% CO2.
      Figure 2:Excitation (dotted line) and emission spectra of SNAP-Cell 505-Star coupled to SNAP-tag in buffer at pH 7.5Figure 2: Excitation (dotted line) and emission spectra of SNAP-Cell 505-Star coupled to SNAP-tag in buffer at pH 7.5
      9147a_thumb
      Figure 3.Structure of SNAP-Cell 505-Star(MW 621.0 g/mol).
      This product is related to the following categories:
      SNAP-tag Substrates Products ,
      Protein Labeling Products
      This product can be used in the following applications:
      SNAP Cell,
      In vivo Imaging,
      Pulse Chase,
      Receptor Internalization,
      Protein Localization,
      Protein Labeling Snap Clip
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