Rabbit polyclonal antibody raised against synthetic peptide of Actin (32 KDa fragment).
Immunogen:
A synthetic peptide corresponding to C-terminus of 32 kDa actin fragment.
Host:
Rabbit
Theoretical MW (kDa):
32
Reactivity:
Human, Rat
Specificity:
This antibody has been directly tested for reactivity in human and rat. specific to the ~ 32 KDa fractin protein in Western blots with no reactivity to intact actin. There is often a ladder of smaller bands in cells or culture or in vivo preparations due to further degradation by other proteases.
Form:
Liquid
Recommend Usage:
Western Blot (1:1000)Immunohistochemistry (1:100)The optimal working dilution should be determined by the end user.
Storage Buffer:
No additive
Storage Instruction:
Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Datasheet:
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Publication Reference
1.
Caspase-3 activation and caspase-like proteolytic activity in human perinatal hypoxic-ischemic brain injury.Rossiter JP, Anderson LL, Yang F, Cole GM.Acta Neuropathol. 2002 Jan;103(1):66-73.
2.
Multiple-label immunocytochemistry for the evaluation of nature of cell death in experimental models of neurodegeneration.Adamec E, Yang F, Cole GM, Nixon RA.Brain Res Brain Res Protoc. 2001 Jul;7(3):193-202.
3.
Inhibition of caspase-3-like activity reduces glutamate induced cell death in adult rat retina.Chen TA, Yang F, Cole GM, Chan SO.Brain Res. 2001 Jun 15;904(1):177-88.
Applications
Western Blot (Cell lysate)
Western blot of colchicine treated SH-SY5Y cell lysate showing specific immunolabeling of the ~ 32k cleaved actin fragment (fractin).
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