Rabbit polyclonal antibody raised against synthetic peptide of CD274.
Immunogen:
A synthetic peptide corresponding to internal region 17 amino acids of human CD274.
Host:
Rabbit
Reactivity:
Human, Mouse, Rat
Specificity:
Has no cross-reactivity to CD273.
Form:
Liquid
Purification:
Peptide affinity purification
Isotype:
IgG
Recommend Usage:
Flow Cytometry (0.5 ug/mL)Immunofluorescence (20 ug/mL)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (2.5 ug/mL)Western Blot (0.5-1 ug/mL)The optimal working dilution should be determined by the end user.
Storage Buffer:
In PBS (0.02% sodium azide).
Storage Instruction:
Store at 4°C for three months. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Datasheet:
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Publication Reference
1.
Hepatic Artery Embolization Enhances Expression of Programmed Cell Death 1 Ligand 1 in an Orthotopic Rat Hepatocellular Carcinoma Model: In Vivo and in Vitro Experimentation.Haruyuki Takaki, Yutaka Hirata, Eisuke Ueshima, Hiroshi Kodama, Seiji Matsumoto, Reona Wada, Hitomi Suzuki, Keiji Nakasho, Koichiro Yamakado.J Vasc Interv Radiol. 2020 Sep;31(9):1475-1482.e2. doi: 10.1016/j.jvir.2020.03.023. Epub 2020 Aug 14.
2.
Immune accessory functions of human endothelial cells are modulated by overexpression of B7-H1 (PDL1).LaGier AJ, Pober JS.Hum Immunol. 2006 Aug;67(8):568-78. Epub 2006 May 19.
3.
Function and regulation of MHC class II molecules in T-lymphocytes: of mice and men.Holling TM, Schooten E, van Den Elsen PJ.Hum Immunol. 2004 Apr;65(4):282-90.
4.
Induced expression of PD-1, a novel member of the immunoglobulin gene superfamily, upon programmed cell death.Ishida Y, Agata Y, Shibahara K, Honjo T.EMBO J. 1992 Nov;11(11):3887-95.
Applications
Western Blot (Cell lysate)
Western blot analysis of CD274 in Raji cell lysate with CD274 polyclonal antibody (Cat # PAB12985) at (A) 0.5 and (B) 1 ug/mL .
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