当前位置 ： Millipore >>> Millipore/MAB351 | Anti-Glutamate Decarboxylase Antibody, 65 kDa isoform, clone GAD-6/MAB351/100 µg
Millipore/MAB351 | Anti-Glutamate Decarboxylase Antibody, 65 kDa isoform, clone GAD-6/MAB351/100 µg
  • Millipore/MAB351 | Anti-Glutamate Decarboxylase Antibody, 65 kDa isoform, clone GAD-6/MAB351/100 µg

Millipore/MAB351 | Anti-Glutamate Decarboxylase Antibody, 65 kDa isoform, clone GAD-6/MAB351/100 µg

价格: ¥4812.00 市场价: 8020.00
打印产品信息
货号: MAB351
品牌: Millipore
规格
数量
库存(0)
特别 提示
代购产品：无质量问题不接受退换货，下单前请仔细核对信息。
下单后请及时联系客服核对商品价格，订单生效后再付款。
立即购买 加入购物车

收藏商品
资深产品顾问
咨询顾问

全国免费服务热线

4000-520-616

  • 自营商城 一站式服务
  • 厂家直采 剔除溢价
  • 品质甄选 正品保证
  • 严控流程 只做188精品
  • 极速物流 如约送货
  • 详情
  • 使用说明
  • 常见问题
    • Description
      CatalogueNumberMAB351
      BrandFamilyChemicon®
      TradeName
      • Chemicon
      DescriptionAnti-GlutamateDecarboxylaseAntibody,65kDaisoform,cloneGAD-6
      AlternateNames
      • GAD65
      BackgroundInformationGutamicaciddecarboxylase(GAD;E.C.4.1.1.15)istheenzymeresponsIBLefortheconversionofglutamicacidtogamma-aminobutyricacid(GABA),themajorinhibitorytransmitterinhigherbrainregions,andputativeparacrinehormoneinpancreaticislets.TwomolecularformsofGAD(65kDaand67kDa,64%aaidentitybetweenforms)arehighlyconservedandbothformsareexpressedintheCNS,pancreaticisletcells,testis,oviductandovary.Theisoformsareregionallydistributedcytoplasmicallyinthebrainsofratsandmice(Sheikh,S.etal.1999).GAD65isanampiphilic,membrane-anchoredprotein(585a.a.),encodedonhumanchromosome10,andisresponsibleforvesicularGABAproduction.GAD67iscytoplasmic(594a.a.),encodedonchromosome2,andseemstoberesponsibleforsignificantcytoplasmicGABAproduction.GADexpressionchangesduringneuraldevelopmentinratspinalcord.GAD65isexpressedtransientlyincommissuralaxonsaroundE13butisdownregulatedthenextdaywhileGAD67expressionincreasesmostlyinthesomataofthoseneurons(Phelps,P.etal.1999).Inmatureratpancreas,GAD65andGAD67appeartobedifferentiallylocalized,GAD65primarilyininsulin-containingbetacellsandGAD67inglucagon-containing(A)cells(Li,L.etal.1995).GAD67expressionseemstobeparticularlyplasticandcanchangeinresponsetoexperimentalmanipulation(forexampleneuronalstimulationortransection)ordiseaseprogressionandemergentdisorderslikeschizophrenia(Volketal.,2000).ColocalizationofthetwoGADisoformsalsoshowschangesinGAD65/GAD67distributionscorrelatedwithcertaindiseasestatessuchasIDDMandSMS.
      ProductInformation
      FormatPurified
      Control
      • Ratbraintissue
      • humanbrainlysate
      PresentationPurifiedimmunoglobulin.Lyophilizedfrom10mMpotassiumphosphate,70mMsodiumchloride,pH7.4,0.02%sodiumazide.Reconstitutewith100μLofsterilewater(1mg/mL).
      StorageandShippingInformation
      StorageConditionsMaintainlyophilizedmaterialat-20°Cforupto12months.Afterreconstitutionmaintainfrozenat-20°Cinundilutedaliquotsforupto6months.Avoidrepeatedfreeze/thawcycles.
      Applications
      ApplicationAnti-GADAntibody.65kDaisoform,cloneGAD-6detectslevelofGlutamateDecarboxylase&hasbeenpublished&validatedforuseinIH&WB.
      KeyApplications
      • Immunohistochemistry
      • WesternBlotting
      ApplicationNotesImmunohistochemistry:(1μg/mL*Seeprotocol)

      Westernblot

      Optimalworkingdilutionsmustbedeterminedbyenduser.

      APPLICATIONNOTESFORMAB351

      IMMUNOHISTOCHEMISTRY

      1)Perfuseratswith100mMphosphatebuffer,pH7.4containing1%paraformaldehyde,0.34%L-lysineand0.05%m-periodate(1%PLP).

      2)Postfixbrainsin1%PLPfor1-2hours.

      3)Transferbrainsto100mMphosphatebuffercontaining30%sucroseandgentlyagitateonashakerplatformat+4°Cfor48-60hours.

      4)Usingaslidingmicrotome,cut30mmsectionsoffrozencerebellum.Asthesectionsarecut,collecttheminavialofcold100mMphosphatebuffer.

      5)IncubatesectionsinPBScontaining1.5%normalserumand0.2%TritonX-100for30minutes.

      6)Onashakerplatform,incubatesectionswithMAB351(diluted1μg/mLinPBScontaining1.5%normalserumand0.2%TritonX-100)for12-36hoursat+4°C.

      7)Onashakerplatform,rinsesectionseighttimes,10-15minutesperrinse,inPBS.

      8)Detectwithstandardsecondaryantibodydetectionsystem(PAP,ABC,etc.).

      9)Mountsections,dehydrate,andapplycoverslips.
      BIOLOGicalInformation
      ImmunogenPurifiedratbrainglutamicaciddecarboxylase.
      CloneGAD-6
      ConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
      HostMouse
      SpecificityRecognizesthelowermolecularweightisoformofthetwoGADisoformsidentifiedinbrain(Gottlieb,etal.,1986;Chang&Gottlieb,1988).Thismonclonalantibodycanbeusedforimmunohistochemicallocalizationinbrainorpancreas.Anti-GADhasalsobeenusedtolabelpurifiedGADonWesternblots(Chang&Gottlieb,1988).
      IsotypeIgG2a
      SpeciesReactivity
      • Human
      • Rat
      AntibodyTypeMonoclonalAntibody
      EntrezGeneNumber
      EntrezGeneSummaryThisgeneencodesoneofseveralformsofglutamicaciddecarboxylase,identifiedasamajorautoantigenininsulin-dependentdiabetes.Theenzymeencodedisresponsibleforcatalyzingtheproductionofgamma-aminobutyricacidfromL-glutamicacid.ApathogenicroleforthisenzymehasbeenidentifiedinthehumanpancreassinceithasbeenidentifiedasanautoantibodyandanautoreactiveTcelltargetininsulin-dependentdiabetes.Thisgenemayalsoplayaroleinthestiffmansyndrome.
      GeneSymbol
      • GAD2
      • MGC161607
      • GAD65
      • MGC161605
      • GAD-65
      • EC4.1.1.15
      PurificationMethodProteinAPurfied
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:Q05329#CatalyzestheproductionofGABA.
      COFACTOR:Pyridoxalphosphate.
      SIZE:585aminoacids;65411Da
      SUBUNIT:Homodimer(Bysimilarity).
      SUBCELLULARLOCATION:Cytoplasm,cytosol.Cytoplasmicvesicle.Celljunction,synapse,presynapticcellmembrane;Lipid-anchor.Golgiapparatusmembrane;Peripheralmembraneprotein;Cytoplasmicside.Note=Associatedtocytoplasmicvesicles.Inneurons,cytosolicleafletofGolgimembranesandpresynapticclusters.
      PTM:Phosphorylated;whichdoesnotaffectkineticparametersorsubcellularlocation.&Palmitoylated;whichisrequiredforpresynapticclustering.
      SIMILARITY:SwissProt:Q05329##BelongstothegroupIIdecarboxylasefamily.
      MolecularWeight65kDa
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
    售后保障
    蚂蚁淘生物188，秉承蚂蚁淘一贯的严谨态度，由蚂蚁淘公司专业人员负责品控、采购、物流、销售、售后，保障正品优质。以“快速好省，为科研提供好产品、好价格”为理念，直接链接原厂家，从全国各地原制造商严格挑选188款科研精品，剔除品牌溢价，188生物新电商，把好的产品带给科研！  力求给你最优质的商品。
  • Q：生物188产品正品保障吗？
    A：生物188质量把控人员具有十年的从业经验，在业界享有良好的口碑；自营商城，直接从厂家采购， 自己的团队负责国际物流和清关，中间没有第三方，所有流程严格把控，100%保证正品，假一罚十。

    Q：下单后可以修改订单吗？
    A：下单后的商品付款之前可以修改；订单付款成功，需要联系我们客服进行修改；客服电话：4000-520-616

    Q：可以开发票吗？
    A：本网站所售商品都是正规清关，均开具16%正规发票，发票金额含配送费金额，另有说明的除外。

    Q：商品几天可以发货？
    A：生物188商品，全部现货销售，付款后即可发货,一般一周内送达！

    Q：如何联系商家？
    A：有任何问题够可以电话咨询我们，全国24小时免费服务热线：4000-520-616 或联系我们的在线客服QQ：1570468124

    Q：收到的商品少了/发错了怎么办？
    A：同个订单购买多个商品可能会分为一个以上包裹发出，可能不会同时送达，建议查看订单详情是否是 部分发货状态；如未收到，可联系在线客服或者致电4000-520-616。

    Q：退换货/维修需要多长时间？
    A：一般情况下，退货处理周期为客户收到产品一个月内（以快递公司显示签收时间为准），包装规格、 数量、品种不符，外观毁损、短缺或缺陷，请在收到货24小时内申请退换货；特殊商品以合同条款为准。

客户服务

在线客服
用户反馈

何为188

极简而严谨，我们仅销售188款生物医学科研用品，款款都是爆款；因为少所以聚焦，聚焦甄选每一款产品，聚焦服务每一位客户!

关注我们 :

点击QQ联系我们
生物188微信

关注188微信公众号

获取最新优惠活动通知

  • 品质甄选，正品保证

  • 自营电商，厂家直采

  • 极简主义，188精品

常见问题 | 购买相关 | 配送与验收 | 支付问题 | 售后与服务 | 服务协议 | 隐私政策 | 何为188 | 中国智造 | 联系我们
友情链接： Applied Biophysics中国 | 供应polysciences | Jellagen价格 | Usbio分子生物学试剂盒 | TEG-Capital产品代理 | Immunostep厂家 | 伯乐代理 | 蚂蚁淘 | 华西实验动物

公司版权所有 © 2005-2021 苏州蚂蚁淘生物科技有限公司 苏ICP备17049038号-10