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Millipore/MAB3418 | Anti-MAP2 Antibody, clone AP20/MAB3418/200 µg
  • Millipore/MAB3418 | Anti-MAP2 Antibody, clone AP20/MAB3418/200 µg

Millipore/MAB3418 | Anti-MAP2 Antibody, clone AP20/MAB3418/200 µg

价格: ¥4812.00 市场价: 8020.00

货号: MAB3418
品牌: Millipore
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    • Description
      CatalogueNumberMAB3418
      BrandFamilyChemicon®
      TradeName
      • Chemicon
      DescriptionAnti-MAP2Antibody,cloneAP20
      AlternateNames
      • MicrotubuleAssociatedProtein
      • MAP2Aand2B
      BackgroundInformationMAP-2(microtubuleassociatedprotein-2)isoneofseveralhighmolecularweightproteinsthatplayanimportantroleinbrainneuronmicrotubuleassembly.Inadditiontoitsassociationwithmicrotubules,MAP-2associateswithneurofilamentsandactinfilamentssuggestingthatitmayguideinteractionamongmicrotubules,othercytoskeletalelements,andcytoplasmicorganelles.MAP-2isastringentMarkerforneurons.Inaddition,MAP-2displaysintracellularspecificity.Inthecentralnervoussystem,MAP-2isconfinedtoneuronalcellbodiesanddendrites.Thereareexceptions,however,wheresomeaxonsstainpositiveforsmallamountsofMAP-2.MAP-2isuniformlydistributedthroughoutthecellwhenfirstexpressedinculturedneuronsbutbecomesselectivelylocalizedasdendriticdevelopmentproceeds.(Caceres,1986;Dotti,1987).
      ProductInformation
      FormatPurified
      Control
      • Brain,Neuronalculture,HumanglioblastomaT98Gcells.
      PresentationPurifiedmousemonoclonalIgG1inbuffercontaining0.02MPhosphatebuffer,pH7.6,0.25MNaClwith0.1%sodiumazide.
      StorageandShippingInformation
      StorageConditionsStablefor6monthsat2-8°Cinundilutedaliquotsfromdateofreceipt.
      Duringshipment,smallvolumesofproductwilloccasionallybecomeentrappedinthesealoftheproductvial.Forproductswithvolumesof200µLorless,werecommendgentlytappingthevialonahardsurfaceorbrieflycentrifugingthevialinatabletopcentrifugetodislodgeanyliquidinthecontainer’scap.
      Applications
      ApplicationAnti-MAP2Antibody,cloneAP20detectslevelofMAP2&hasbeenpublished&validatedforuseinIH&WB.
      KeyApplications
      • Immunohistochemistry
      • WesternBlotting
      ApplicationNotesWesternBlot:
      60ng/mL.InSDS-PAGE,MAP-2fromadultratmigratesasacloselyassociateddoublethavingamolecularweightofapproximately280-300kDrepresentingtheMAP-2Aand-2Bisoformsbutnotthe70kDaMAP-2Cisoform.However,earlyinbraindevelopment(postnatalday10inrats),MAP-2migratesasasinglebandthatisidenticaltothelowermolecularweightbandoftheadultMAP-2doublet(MAP-2b).Laterindevelopment(postnataldays17-18),themobilityofMAP-2changestotheadultdoubletform.(Inthespinalcord,conversiontotheadultformoccursearlier).Usersshouldrun4%-20%SDS-PAGEgrADIentgels.

      Immunohistochemistry:
      5µg/mL(4%PFAfixedfrozensections).

      Immunohistochemistry:
      5µg/mLofapreviouslotwasused.Anti-MAP-2canbeusedtostaintissue(brainorspinalcord)fixedwithparaformaldehyde.

      Optimalworkingconcentrationsmustbedeterminedbytheenduser.
      BIOLOGicalInformation
      ImmunogenBovinebrainmicrotubuleprotein.
      CloneAP20
      ConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
      HostMouse
      SpecificityMAB3418bindsspecificallytoMAP-2.
      IsotypeIgG
      SpeciesReactivity
      • Bovine
      • Chicken
      • Human
      • Mouse
      • Rat
      • Xenopus
      • Quail
      SpeciesReactivityNoteRatandmouse.Expectedtocross-reactwithhuman,cow,chicken,quail,andXenopus.
      AntibodyTypeMonoclonalAntibody
      EntrezGeneNumber
      EntrezGeneSummaryThisgeneencodesaproteinthatbelongstothemicrotubule-associatedproteinfamily.Theproteinsofthisfamilyarethoughttobeinvolvedinmicrotubuleassembly,whichisanessentialstepinneurogenesis.Theproductsofsimilargenesinratandmouseareneuron-specificcytoskeletalproteinsthatareenrichedindentrites,implicatingaroleindeterminingandstABIlizingdentriticshapeduringneurondevelopment.Anumberofalternativelysplicedvariantsencodingdistinctisformshavebeendescribed.
      GeneSymbol
      • MAP2
      • MAP2B
      • MAP-2
      • MAP2C
      • DKFZp686I2148
      • MAP2A
      PurificationMethodProteinAPurfied
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:P11137#TheexactfunctionofMAP2isunknownbutMAPsmaystabilizethemicrotubulesagainstdepolymerization.Theyalsoseemtohaveastiffeningeffectonmicrotubules.
      SIZE:1827aminoacids;199539Da
      PTM:MAP2A/cisphosphorylated.PhosphorylateduponDNAdamage,probablybyATMorATR.
      SIMILARITY:SwissProt:P11137##Contains3Tau/MAPrepeats.
      MolecularWeight230kDa
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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