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Millipore/HTS002M | ChemiScreen™ CXCR2 Membrane Preparation/HTS002M/200 assays
  • Millipore/HTS002M | ChemiScreen™ CXCR2 Membrane Preparation/HTS002M/200 assays

Millipore/HTS002M | ChemiScreen™ CXCR2 Membrane Preparation/HTS002M/200 assays

价格: ¥7152.00 市场价: 11920.00

货号: HTS002M
品牌: Millipore
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    • Description
      CatalogueNumberHTS002M
      BrandFamilyChemicon®
      TradeName
      • ChemiScreen
      • Chemicon
      DescriptionChemiScreen™CXCR2MembranePreparation
      OverviewTRANSFECTION:Full-lengthhumanCXCR2CDNA(AccessionNumber:M73969)
      BackgroundInformationCXCR2isa7-TMG-proteincoupledreceptorthatbindstothechemokinesGROalpha,GRObeta,GROgamma,IL-8,ENA-78,NAP-2andGCP-2(OlsonandLey,2002).Neutrophils,mastcellsandmicrovascularendothelialcellsexpressCXCR2,andinteractionsofCXCR2withitsligandspromoteschemotaxisofthesecelltypes(Heidemannetal.,2003;Nilssonetal.,1999;Whiteetal.,1998).StudieswithmicelackingCXCR2indicatethatCXCR2promotesgrowthofprimarytumorsandsecondarymetastases(Keaneetal.,2004),andplaysanessentialroleinhyperoxia-inducedlunginjury(Sueetal.,2004).Inaddition,cytomegalovirusencodesaCXCR2-bindingchemokine,vCXC-1,thatpromotesneutrophilmigrationtoinfectedcells(Penfoldetal.,1999).Chemicon"sCXCR2MembranePrepsareidealtoolsforscreeningforantagoNISTsofinteractionsbetweenCXCR2anditsligands.RADIolabeledIL-8bindstotheCXCR2membraneswithaKdof0.075nM.With0.1nM125I-labeledIL-8,a5-foldwindowofspecificbindingisobtained.
      ProductInformation
      FormatMembranes
      PresentationMembraneswereadjustedto0.5mgprotein/mlin50mMTrispH7.4,10%glyceroland1%BSA,rapidlyfrozen,andstoredat-80°C.Onevialtypicallycontains1000units,whereaunitistheamountofmembranethatwillyieldgreaterthan1000cpmspecificbindingof125I-labeledIL-8(0.1nMinputconcentration).
      StorageandShippingInformation
      StorageConditionsMaintainfrozenat-70°Cforupto2years.Donotfreezeandthaw.
      Applications
      ApplicationHumanCXCR2/IL-8bGPCRmembranepreparationforRadioligandbindingAssays.
      KeyApplications
      • Radioligandbindingassay
      ApplicationNotesRadioligandbindingassay.
      BIOLOGicalInformation
      Concentration0.5mg/mL
      EntrezGeneNumber
      EntrezGeneSummaryTheproteinencodedbythisgeneisamemberoftheG-protein-coupledreceptorfamily.Thisproteinisareceptorforinterleukin8(IL8).ItbindstoIL8withhighaffinity,andtransducesthesignalthroughaG-proteinactivatedsecondmessengersystem.Thisreceptoralsobindstochemokine(C-X-Cmotif)ligand1(CXCL1/MGSA),aproteinwithmelanomagrowthstimulatingactivity,andhasbeenshowntobeamajorcomponentrequiredforserum-dependentmelanomacellgrowth.Thisreceptormediatesneutrophilmigrationtositesofinflammation.TheangiogeniceffectsofIL8inintestinalmicrovascularendothelialcellsarefoundtobemediatedbythisreceptor.Knockoutstudiesinmicesuggestedthatthisreceptorcontrolsthepositioningofoligodendrocyteprecursorsindevelopingspinalcordbyarrestingtheirmigration.Thisgene,IL8RA,ageneencodinganotherhighaffinityIL8receptor,aswellasIL8RBP,apseudogeneofIL8RB,formageneclusterinaregionmappedtochromosome2q33-q36.
      GeneSymbol
      • IL8RB
      • CD182
      • CMKAR2
      • CXCR-2
      • IL8RA
      • IL8R2
      • CXCR2
      • CDw128b
      GPCRClassA
      HostCellsChem-1
      ProteinTargetCXCR2/IL-8b
      TargetSub-FamilyChemokine
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:P25025#Receptortointerleukin-8,whichisapowerfulneutrophilschemotacticfactor.BindingofIL-8tothereceptorcausesactivationofneutrophils.ThisresponseismediatedviaaG-proteinthatactivateaphosphatidylinositol-calciumsecondmessengersystem.ThisreceptorbindstoIL-8withahighaffinityandtoGRO/MGSAandNAP-2alsowithahighaffinity.
      SIZE:360aminoacids;40759Da
      SUBCELLULARLOCATION:Cellmembrane;Multi-passmembraneprotein.
      PTM:Phosphorylateduponligandbinding;whichisrequiredfordesensitization.
      SIMILARITY:SwissProt:P25025##BelongstotheG-proteincoupledreceptor1family.
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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