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Millipore/HTH17MAG-14K | MILLIPLEX <small>MAP</small> Human TH17 Magnetic Bead Panel - Immunology Multiplex Assay/HTH17M
  • Millipore/HTH17MAG-14K | MILLIPLEX <small>MAP</small> Human TH17 Magnetic Bead Panel - Immunology Multiplex Assay/HTH17M

Millipore/HTH17MAG-14K | MILLIPLEX MAP Human TH17 Magnetic Bead Panel - Immunology Multiplex Assay/HTH17M

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货号: HTH17MAG-14K
品牌: Millipore
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    • Description
      CatalogueNumberHTH17MAG-14K
      TradeName
      • MILLIPLEX
      DescriptionMILLIPLEXMAPHumanTH17MagneticBeadPanel-ImmunologyMultiplexAssay
      OverviewEMDMillipore’sMILLIPLEX®MAPHumanTH17MagneticBeadPanelisa25-plexkittobeusedforthesimultaneousquantificationofthefollowinganalytesinserum,plasmaorculturesupernatantsamples:GM-CSF,IFN-γ,IL-1β,IL-2,IL-4,IL-5,IL-6,IL-9,IL-10,IL-12(p70),IL-13,IL-15,IL-17A,IL-17F,IL-17E/IL-25,IL-21,IL-22,IL-23,IL-27,IL-28A,IL-31,IL-33,MIP-3α/CCL20,TNF-αandTNFβ.

      MILLIPLEX®MAPoffersthebroadestselectionofanalytesacrossawiderangeofdiseasestatesandspecies.Oncetheanalytesofinteresthavebeenidentified,youcanrelyonthequalitythatwebuildintoeachkittoproduceresultsyoucantrust.Inadditiontotheassaycharacteristicslistedintheprotocol,otherperformancecriteriaevaluatedduringthevalidationprocessinclude:cross-reactivity,dilutionlinearity,kitstABIlityandsamplebehavior(e.g.detectabilityandstability).Eachpanelandkitmeetsstringentmanufacturingcriteriatoensurebatch-to-batchreproducibility.

      EachMILLIPLEX®MAPpanelandkitincludes:
      •Qualitycontrols(QCs)providedtoqualifyassayperformance

      •Comparisonofstandard(calibrator)andQClotstoareferencelottoensurelot-to-lotconsistency

      •Optimizedserummatrixtomimicnativeanalyteenvironment

      •Detectionantibodycocktailsdesignedtoyieldconsistentanalyteprofileswithinpanel


      MILLIPLEX®MAPoffersyou:
      oTheabilitytochooseanycombinationofanalytesfromourpanelofsevenanalytestodesignacustomkitthatbettermeetsyourneeds.
      oAconvenient“all-in-one”boxformatthatgivesyoutheassurancethatyouwillhaveallthenecessaryreagentsyouneedtorunyourassay.

      EMDMillipore’sMILLIPLEX®MAPHumanTH17MagneticBeadPanelispartofthemostversatilesystemavailableforcomplementfactorresearch.FromoursingletomultiplexbioMarkersolutions,wepartnerwithyoutodesign,develop,analyticallyvalidateandbuildthemostcomprehensivelibraryavailableforproteindetectionandquantitation.
      BackgroundInformationCD4T-helpercellsaremajorplayersinadaptiveimmunity.Basedontheirexpressionprofileoftranscriptionfactorsandsecretedcytokines,thesecellsaredividedintothemajorsubsetsTh1,Th2,Th17andTregulatory(Treg)cells.Th1cells,inducedbyIL-12andenhancedbyIFNγ,secreteIFNγthatactivatesmacrophages.InducedbyIL-4,Th2cellsprimarilyproduceIL-4,IL-5,IL-13,IL-25andIL-17E,playingaroleinallergy.Tregcells,inducedbyTGFβ,producetheanti-inflammatorycytokinesIL-10andTGFβandfunctiontocontrolT-cellresponsestopreventautoimmunereactions.Th17cells,amorerecentlydiscoveredsubsetcharacterizedbytheproductionofIL-17,areinducedbyTGFβcombinedwithIL-23andIL-1βinhumans,andTGFβ,IL-6andIL-21inmice.ActivatedTh17cellssecreteIL-17A,IL-17F,IL-21,IL-22andTNFαtopromotetissueinflammation.

      Th17cellsareinvolvedintheclearanceofextracellularbacteriaandfungi.TheyareabundantintheintestinallaminapropriaandfunctionasabarrieragainstinvADIngpathogens.ExcessiveamountsofTh17cellshavebeenimplicatedinthepathogenesisofseveralautoimmunediseases,includingmultiplesclerosis,psoriasis,juvenilediabetes,rheumatoidarthritis,Crohn"sdiseaseandautoimmuneuveitis.InadditionTh17cellsplayanimportantroleintumordevelopment,progressionandmetastasis,potentiallyinbothpromotingandinhibitingtumorgrowth.

      TheMILLIPLEX®MAPHumanTH17MagneticBeadPanellenablesyoutofocusontherelevanceandtherapeuticpotentialofthemodulationofCD4T-helpercellcytokinesinyourresearchmodel.CoupledwiththeLuminexxMAP®platforminamagneticbeadformat,youreceivetheadvantageofidealspeedandsensitivity,allowingquantitativemultiplexdetectionofmultipleanalytessimultaneously,whichcandramaticallyimproveproductivity.
      ProductInformation
      DetectionmethodLuminexxMAP
      ConfigurationDesignyourmultiplexkitbychoosingavailableanalyteswithinthispanel.
      Precision,%
      • Inter-assay:<>
      • Intra-assay:<>
      PanelTypeMAGNETICCytokines/Chemokines
      StorageandShippingInformation
      StorageConditionsRecommendedstorageforkitcomponentsis2-8°C.
      Applications
      ApplicationSimultaneouslyanalyzemultipleTh17cytokineandchemokinebiomarkerswiththeTh17Bead-BasedMultiplexAssaysusingtheLuminextechnology,inhumanserum,plasmaandcellculturesamples.
      KeyApplications
      • Multiplexing
      ApplicationNotes•Thisisanovernightincubationassayrequiring25µLofneatplasmaorserum,or25μLcellculturesupernatantperwell.
      •ThiskitmaybeusedfortheanalysisofalloranycombinationoftheTh17panelanalytesinserum,plasmaortissue/celllysateandculturesupernatant
      BIOLOGicalInformation
      SpeciesReactivity
      • Human
      AnalytesAvailable
      • GM-CSF
      • IFN-γ
      • IL-1β
      • IL-2
      • IL-4
      • IL-5
      • IL-6
      • IL-9
      • IL-10
      • IL-12(p70)
      • IL-13
      • IL-15
      • IL-17A
      • IL-17F
      • IL-17E/IL-25
      • IL-21
      • IL-22
      • IL-23
      • IL-27
      • IL-28A
      • IL-31
      • IL-33
      • MIP-3α/CCL20
      • TNF-α
      • TNFβ
      CrossReactivityCross-reactivitybetweentheantibodiesandanyoftheotheranalytesinthispanelisnon-detectableornegligIBLe.
      Accuracy
      • 79-107%
      PhysicochemicalInformation
      Sensitivity
      • Refertokitprotocolforsensitivitiesofindividualbiomarkers.
      StandardCurveRange
      • IL-17&IL-4,24-100,000pg/mL
      • GM-CSF&IL-27,61-250,000pg/mL
      • IFNγ,10-40,000pg/mL
      • IL-10,1-5000pg/mL
      • MIP-3α,IL-1β,IL-12(p70),IL-15,IL-21&IL-33,5-20,000pg/mL
      • IL-13,7-30,000pg/mL
      • IL-17A&IL-2,12-50,000pg/mL
      • IL-22&TNFβ,37-150,000pg/mL
      • IL-9,9-35,000pg/mL
      • IL-23,336-1,500,000pg/mL
      • IL-5,6-25,000pg/mL
      • IL-6&TNFα,3-10,000pg/mL
      • IL-17E/IL-25,488-2,000,000
      • IL-28a&IL-31,49-200,000pg/mL
      Dimensions
      MaterialsInformation
      MaterialsInformation
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