1XHelicaseBufferM: 10mMDithiolthreitol 10mMMagnesiumAcetate 35mMTris-AcetatepH7.5@25°C
3mMATP. Incubateat37ºC.
Notes: E.coliUvrDhelicasemayrequireMutLand/orSSBforoptimalperformanceinspecificapplications.
:MutLstimulatesUvrDhelicaseactivitymorethantenfoldbyloADIngitontotheDNAsubstrate(MechanicLE,FrankelBA,MatsonSW(2000)JBiolChem275:3833738346).
:MutLandSSBarerequiredinHelicase-DependentAmplificationusingE.coliUvrD(MyriamVincent,YanXu,andHuiminKong(2004)EMBOreportsVol.5,795-800).
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