TheTruSeqSyntheticLong-ReadDNALibraryPrepKitisahighlyaccurate,end-to-endsequencingsolutionthatcanbeusedforgenomeassemblyorgenomephasing.Dependingontheanalysisoptionyouselect,youcanuseitto:
- Assemblesyntheticallylongreadsfordenovoassemblyandgenomefinishingapplications
- Performgenomephasingtoidentifyco-inheritedallelesandhaplotypeinformation,aswellasphasedenovomutations
AccuratelyConstructSyntheticLongReads
TheTruSeqSyntheticLong-ReadDNALibraryPrepandTruSeqSyntheticLong-ReadDNABarcodeKitsaredesignedforpreparingDNAlibrariestogeneratesyntheticallylongreads.ThelibraryprepkitcombinesTruSeqandNexterachemistrieswithsyntheticlong-readtechnologytoprepareDNAlibraries.Theaccompanyingbarcodekitincludes384indexesforlabelingthesamplesineachwell.Theseindexesarethenusedaftersequencingtoconstructsyntheticallylongfragmentsforlong-readassemblyandphasinganalysis.
SimplifiedInformaticsforGenomeAssemblyorHumanWhole-GenomePhasing
Aftersequencing,push-buttonanalysisinBaseSpaceSequenceHubsimplifiesassemblyoflongreads.DatacanbetransferredfromanIlluminasequencinginstrumenttotheBaseSpaceSequenceHubcloudinstantly.
BaseSpaceAppforGenomeAssembly:
TheTruSeqLong-ReadAssemblyAppconstructslong,syntheticreadsfromshortersequencingreadsforaccurategenomeassemblyandgenomefinishing.TruSeqsyntheticlong-readtechnologyallowsyoutousethesamefamiliarplatformwithanewapplication.
BaseSpaceAppforPhasingAnalysis:
TheTruSeqPhasingAnalysisAppcanbeusedtoperformwholehumangenomephasing,identifyinghaplotypeinformationandco-inheritedalleles,andphasingdenovomutations.Byconstructingsyntheticallylongfragmentsfromshortersequencingreads,thismethodprovidesmorecomprehensiveandaccuratephasingcomparedtoconventionaltriostudiesorstatisticalinference.