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NEB/NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina®/E7420L/24 reactions
  • NEB/NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina®/E7420L/24 reactions

NEB/NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina®/E7420L/24 reactions

价格: ¥13860.00 市场价: 23100.00

品牌: NEB
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    • Description:







      TheNEBNext®Ultra™ Directional RNALibraryPrepKitforIllumina®containsenzymesandbuffersthatareideallysuitedforCDNAlibrarypreparationfornext-generationsequencing.Eachofthesecomponentsmustpassrigorousqualitycontrolstandardsandarelotcontrolled,bothindividuallyandasasetofreagents.

      UltraDirectionalRNALibraryPreparationforIlluminaUltra Direct

      NEBNextUltra™DirectionalRNAproduceslibraries withhighdirectionality/strandspecificityNEBNext Ultra™ Directional RNA produces libraries with high directionality/strand specificity

      UltraDirectionalRNAlibrariesofmRNApurifiedfromUniversalHumanReferenceRNA(Agilent® #740000),weresequencedontheIlluminaplatform.Thehighpercentageofreadsmappedtothe expectedstrandshowsthehighstrand-specificityofthelibraries.ActinomycinDisrequiredto achievemaximalstrandspecificity.

      NEBNextUltra™DirectionalRNAlibrariesprovideeven coverageacrosstranscriptsNEBNext Ultra™ Directional RNA libraries provide even coverage across transcripts

      mRNAwasisolatedfromUniversalHumanReferenceRNA(Agilent#740000)andlibrariesweremade usingtheNEBNextUltra™DirectionalRNAKitandtheIlluminaTruSeqStrandedmRNAKit.Libraries weresequenced(singleendreads)ontheIlluminaplatform(GAIIx).Goodcoverageacrossexpressed transcriptswasobtained.

      LowinputNEBNextUltraDirectionalRNAlibraries retaincomplexityLow input NEBNext Ultra Directional RNA libraries retain complexity

      RibosomalRNA-depletedRNAlibrariesweremade from10ngor100ngUniversalHumanReferenceRNA (Agilent#740000)usingtheNEBNextUltraDirectional RNAKit.TranscriptabundancesinFragmentsPer KilobaseofexonperMillionfragmentsmapped (FPKM)showstrongconcordance(R2=0.90745), indicatingretentionoflibrarycomplexityevenatlow inputamount.

      Equivalenttranscriptrepresentationisachieved betweentechnicalreplicatesofNEBNextUltra DirectionalRNAlibrariesEquivalent transcript representation is achieved between technical replicates of NEBNext Ultra Directional RNA libraries

      TechnicalreplicatesofmRNAlibrariesmadefrom mRNAisolatedfromUniversalHumanReference RNA(Agilent#740000)showstrongconcordance (R2=0.99409).


      Sequencingdatashowsstrongconcordancebetween NEBNextUltraDirectionalRNAandIllumina TruSeqStrandedRNAlibrariesSequencing data shows strong concordance between NEBNext Ultra Directional RNA and Illumina TruSeq Stranded RNA libraries

      mRNAwasisolatedfromUniversalHumanReference RNA(Agilent#740000)andlibrariesweremadeusing theNEBNextUltraDirectionalRNAKitandtheIllumina TruSeqStrandedmRNAKit.ThisFPKMconcordance plotshowscomparabletranscriptrepresentation (R2=0.96009)betweenthelibraries.


      FunctionalValidation
      EachsetofreagentsisfunctionallyvalidatedtogetherthroughconstructionandsequencingofanRNAlibraryontheIlluminasequencingplatform.

      LotControl

      Thelotsprovidedaremanagedseparatelyandqualifiedbyadditionalfunctionalvalidation.Individualreagentsundergostandardenzymeactivityandqualitycontrolassays,andalsomeetstringentcriteriaintheadditionalqualitycontrolslistedoneachindividualcomponentpage

      ReagentsSupplied

      Thefollowingreagentsaresuppliedwiththisproduct:

      Storeat(°C)Concentration
      NEBNextFirstStrandSynthesisReactionBuffer-20
      RandomPrimers-20
      ProtoScript®IIReverseTranscriptase-20200,000units/ml
      MurineRNaseInhibitor-20
      NEBNextSecondStrandSynthesisEnzymeMix-20
      NEBNextSecondStrandSynthesisReactionBufferwithdUTPMix-2010X
      NEBNextEndPrepEnzymeMix-20
      NEBNextEndRepairReactionBuffer-2010X
      Blunt/TALigaseMasterMix-20
      Nuclease-freeWater-20
      NEBNextUSER®Enzyme-20
      NEBNext®Q5®HotStartHiFiPCRMasterMix-20
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