当前位置 : NEB >>> NEB/LunaScript® RT Master Mix Kit (Primer-free)/25 reactions/E3025S
NEB/LunaScript® RT Master Mix Kit (Primer-free)/25 reactions/E3025S
  • NEB/LunaScript® RT Master Mix Kit (Primer-free)/25 reactions/E3025S

NEB/LunaScript® RT Master Mix Kit (Primer-free)/25 reactions/E3025S

价格: 试用 市场价: 0.00

货号: E3025S
品牌: NEB
规格
数量
库存(0)
特别 提示
代购产品:无质量问题不接受退换货,下单前请仔细核对信息。
下单后请及时联系客服核对商品价格,订单生效后再付款。
资深产品顾问
咨询顾问

全国免费服务热线

4000-520-616


  • 自营商城 一站式服务
  • 厂家直采 剔除溢价
  • 品质甄选 正品保证
  • 严控流程 只做188精品
  • 极速物流 如约送货
  • 详情
  • 使用说明
  • 常见问题
    • The LunaScript RT Master Mix Kit (Primer-free) includes an optimized 5X master mix containing all the necessary components for first strand cDNA synthesis, except primers. The mix features the thermostable Luna Reverse Transcriptase, which supports cDNA synthesis at elevated temperatures. Murine RNase Inhibitor is included to protect template RNA from degradation, along with dNTPs. In addition, the presence of a blue tracking dye provides a visual indicator for the RT step and downstream applications.The mix is compatible with random primers, oligo dT primers, and gene-specific primers, enabling maximum cDNA synthesis flexibility. The cDNA product generated by the LunaScript RT Master Mix (Primer-free) can be used in a variety of downstream applications. For real-time PCR quantitation, a mixture of random and dT primers is recommended to generate cDNA. The mix offers robust, linear and sensitive detection from 1 μg total RNA input down to low copies of RNA, similar to the LunaScript RT SuperMix Kit (NEB #E3010). To generate long or full-length cDNAs, oligo dT primers should be used. With a short incubation at 55°C for 10 minutes, the LunaScript RT Master Mix (Primer-free) can make cDNA products up to 9 kb. A No-RT control mix is provided for ease of setting up control reactions.
      Figure 1: Comparison of supermix, master mix and cDNA synthesis kit formats
      Figure 2:At just 13 minutes, LunaScript offers the shortest available first-strand cDNA synthesis protocol
      Comparison of recommended protocols for cDNA synthesis. Both LunaScript RT SuperMix Kit and LunaScript RT Master Mix Kit require the shortest reaction time and tolerate elevated temperatures, reducing complications from RNA secondary structure.
      Figure 3:LunaScript RT Master Mix (Primer-free) supports numerous applications
      By adding different primers including a Random Primer Mix, d(T))23VN oligos, or random hexamers, the LunaScript RT Master Mix can produce cDNA that is ideally suited for downstream applications such as RT-qPCR, RT-PCR, and RNA-seq studies.
      Figure 4:LunaScript RT Master Mix (Primer-free) offers robust 2-step RT-qPCR quantitation
      A. In the presence of Random Primer Mix (NEB #S1330), the LunaScript RT Master Mix (Primer-free) can deliver linear cDNA yields across RNA inputs from 1 µg to 100 pg with linearity similar to LunaScript (NEB #E3010) in a 2-step RT-qPCR workflow.
      B. For average transcripts, the use of d(T)23VN primers offers cDNA coverage similar to the Random Primer Mix. However, for long transcripts (e.g., SMG1), random primers often enable better coverage of the 5´ end of the target, as seen in the example in the lower righthand box where the RT-qPCR target was located at the 5´ end of the 16 kb SMG1 transcript; its detection by an anchored dT primer alone [d(T)23VN] gave delayed Cq values when compared to the performance using the Random Primer Mix (a mixture of random hexamers and anchored dT primer).
      Figure 5:Better performance of LunaScript RT Master Mix (Primer-free) in 2-step RT-qPCR quantitation
      A. Several first strand cDNA synthesis kits were used according to manufacturer’s recommendations to generate cDNA using the random primers provided with each product. Input included human total RNA from 1 µg – 100 pg. cDNA products were then evaluated by qPCR using eight targets varying in abundance, length and %GC. qPCR detection of the cDNA products was performed using the Luna Universal Probe qPCR Master Mix (NEB #M3004). Results were evaluated for efficiency and ΔCq, where ΔCq measures low input detection and lack of non-template control (NTC) amplification (ΔCq = average Cq of NTC - average Cq of lowest input). Green box indicates target performance criteria (Efficiency = 90–110%, ΔCq ≥ 3). More details on this visualization method can be found in the application note “Development of a high-throughput data analysis method for quantitative real-time PCR (qPCR).”
      B. Results from four targets above (two typical control genes and two others) were plotted to examine Cq as a function of input. LunaScript gave the earliest Cq values when compared to other commercial kits.
      Figure 6:LunaScript RT Master Mix (Primer-free) can generate a broad range of cDNA products for use in routine or high fidelity PCR
      LunaScript RT Master Mix (Primer-free) was used to synthesize cDNA from 1 µg of Jurkat total RNA in the presence of d(T))23VN primers under the standard reaction condition of 55°C for 10 min, 95°C for 1 min. Subsequent to cDNA synthesis, different PCR amplification reagents can be used for PCR detection. For routine application up to 5 kb, OneTaq 2X Master Mix (NEB #M0482) is recommended. For highest fidelity, Q5 Hot Start High-Fidelity 2X Master Mix (NEB #M0494) is recommended. For highest yield of long PCR amplification, LongAmp Taq 2X Master Mix (NEB #M0287) can be used (data not shown).
      This product is related to the following categories:
      Luna® qPCR & RT-qPCR Products,
      RT-PCR Products,
      Reverse Transcriptases & RT-PCR Products,
      cDNA Synthesis & Reverse Transcriptases Products
      This product can be used in the following applications:
      RT-PCR,
      cDNA Synthesis,
      Reverse Transcription (cDNA Synthesis),
      RT-PCR & cDNA Synthesis
    售后保障
    蚂蚁淘生物188,秉承蚂蚁淘一贯的严谨态度,由蚂蚁淘公司专业人员负责品控、采购、物流、销售、售后,保障正品优质。以“快速好省,为科研提供好产品、好价格”为理念,直接链接原厂家,从全国各地原制造商严格挑选188款科研精品,剔除品牌溢价,188生物新电商,把好的产品带给科研!  力求给你最优质的商品。
  • Q:生物188产品正品保障吗?
    A:生物188质量把控人员具有十年的从业经验,在业界享有良好的口碑;自营商城,直接从厂家采购, 自己的团队负责国际物流和清关,中间没有第三方,所有流程严格把控,100%保证正品,假一罚十。

    Q:下单后可以修改订单吗?
    A:下单后的商品付款之前可以修改;订单付款成功,需要联系我们客服进行修改;客服电话:4000-520-616

    Q:可以开发票吗?
    A:本网站所售商品都是正规清关,均开具16%正规发票,发票金额含配送费金额,另有说明的除外。

    Q:商品几天可以发货?
    A:生物188商品,全部现货销售,付款后即可发货,一般一周内送达!

    Q:如何联系商家?
    A:有任何问题够可以电话咨询我们,全国24小时免费服务热线:4000-520-616 或联系我们的在线客服QQ:1570468124

    Q:收到的商品少了/发错了怎么办?
    A:同个订单购买多个商品可能会分为一个以上包裹发出,可能不会同时送达,建议查看订单详情是否是 部分发货状态;如未收到,可联系在线客服或者致电4000-520-616。

    Q:退换货/维修需要多长时间?
    A:一般情况下,退货处理周期为客户收到产品一个月内(以快递公司显示签收时间为准),包装规格、 数量、品种不符,外观毁损、短缺或缺陷,请在收到货24小时内申请退换货;特殊商品以合同条款为准。

何为188

极简而严谨,我们仅销售188款生物医学科研用品,款款都是爆款;因为少所以聚焦,聚焦甄选每一款产品,聚焦服务每一位客户!

关注我们 :

点击QQ联系我们
生物188微信

关注188微信公众号

获取最新优惠活动通知
  • 品质甄选,正品保证

  • 自营电商,厂家直采

  • 极简主义,188精品