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Millipore/CC127 | Caspase 2, recombinant human active/CC127/25 units
  • Millipore/CC127 | Caspase 2, recombinant human active/CC127/25 units

Millipore/CC127 | Caspase 2, recombinant human active/CC127/25 units

价格: ¥3576.00 市场价: 5960.00

货号: CC127
品牌: Millipore
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    • Description
      CatalogueNumberCC127
      BrandFamilyChemicon®
      TradeName
      • Chemicon
      DescriptionCaspase2,recombinanthumanactive
      OverviewCaspase-2(alsoknownasICH-1,NEDD-2)isamemberoftheinterleukin-1betaconvertingenzyme(ICE)familyofcysteinproteases.Similarasothercaspases,caspase-2alsoexistsincellsasaninactiveproenzyme.Duringapoptosis,procaspase-2isprocessedataspartateresiduesbyself-proteolysisand/orcleavagebyupstreamcaspases.Theprocessedformofcaspase-2consistsoflarge(19kDa)andsmall(12kDa)subunitswhichassociatetoformtheactiveenzyme.Theactiverecombinanthumancaspase-2wasexpressedinE.coli.Theexpressedcaspase-2spontaneouslyundergoesautoprocessingtoyieldthesubunitscharacteristicofthenativeenzyme.Therecombinantcaspase-2preferentiallycleavesthesubstratesconsistingofconsensussequenceVDVAD(e.g.,VDVAD-AFCandVDVAD-pNA).Therecombinantenzymecanalsobeusedasapositivecontrolincaspaseassaysorindeterminingthespecificityofcaspasesubstrates.Recommendedusage:0.5-1unitforfluorometriccaspaseassaysand2-4unitsforcolorimetriccaspaseassays.Optimalworkingdilutionsmustbedeterminedbyenduser.
      ProductInformation
      PresentationPartiallypurifiedrecombinantcaspase2withafulllengthHIS-6-tag.Approximately10-20%caspase2protein.TheremainderisE.Coliproteins.Approximately0.8-2mgtotalprotein/Uofactivity;E.Coliproteinshavenoreactivity.Lyophilized.Reconstitutein25μLPBScontaining15%glycerol.
      StorageandShippingInformation
      StorageConditionsMaintainunopenedandrehydratedproductat-70°Cinundiluted,freeze-thawprotected,tightlysealedaliquots.Avoidrepeatedfreeze/thawcycles.Productstablefor6monthsfromdateofpurchasedifhandledappropriately.
      Applications
      KeyApplications
      • ActivityAssay
      BIOLOGicalInformation
      PurityPartiallypurifiedrecombinantcaspase3withafulllengthHIS-6-tag.Approximately10-20%caspase2protein.TheremainderisE.Coliproteins.Approximately0.8-2mgtotalprotein/Uofactivity;E.Coliproteinshavenoreactivity.
      SpecificActivity25U:Whereoneunitoftherecombinantcaspase-2istheenzymeactivitythatcleaves1nmolofthecaspasesubstrateVDVAD-pNA(pNA:pnitroanaline)perhourat37oCinareactionsolutioncontaining50mMHepes,pH7.2,50mMNaCl,0.1%Chaps,10mMEDTA,5%Glycerol,and10mMDTT.
      EntrezGeneNumber
      EntrezGeneSummaryThisgeneencodesaproteinwhichisamemberofthecysteine-asparticacidprotease(caspase)family.Sequentialactivationofcaspasesplaysacentralroleintheexecution-phaseofcellapoptosis.Caspasesexistasinactiveproenzymeswhichundergoproteolyticprocessingatconservedasparticresiduestoproducetwosubunits,largeandsmall,thatdimerizetoformtheactiveenzyme.Theproteolyticcleavageofthisproteinisinducedbyavarietyofapoptoticstimuli.Alternativesplicingofthisgeneresultsinmultipletranscriptvariantsthatencodedifferentisoforms.
      GeneSymbol
      • CASP2
      • ICH1
      • NEDD2
      • NEDD-2
      • ICH-1L/1S
      • CASP-2
      • ICH-1L
      • EC3.4.22.55[Contains:Caspase-2subunitp18
      • Caspase-2subunitp13
      • Caspase-2subunitp12].
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:P42575#InvolvedintheactivationcascadeofcaspasesresponsIBLeforapoptosisexecution.Mightfunctionbyeitheractivatingsomeproteinsrequiredforcelldeathorinactivatingproteinsnecessaryforcellsurvival.
      SIZE:452aminoacids;50685Da
      SUBUNIT:Heterotetramerthatconsistsoftwoanti-parallelarrangedheterodimers,eachoneformedbyap18subunitandap12subunit.InteractswithLRDD.
      TISSUESPECIFICITY:Expressedinlargeramountsintheembryoniclung,liverandkidneythanintheheartandbrain.Intheadultshigherlevelexpressionisseenintheplacenta,lung,kidney,pancreasthanintheheart,brain,liverandskeletalmuscle.
      PTM:Thematureproteasecanprocessitsownpropeptide,butnotthatofothercaspases.
      SIMILARITY:SwissProt:P42575##BelongstothepeptidaseC14family.&Contains1CARDdomain.
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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