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Millipore/CC126 | Caspase 1, recombinant human active/CC126/25 units
  • Millipore/CC126 | Caspase 1, recombinant human active/CC126/25 units

Millipore/CC126 | Caspase 1, recombinant human active/CC126/25 units

价格: ¥3204.00 市场价: 5340.00

货号: CC126
品牌: Millipore
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    • Description
      CatalogueNumberCC126
      BrandFamilyChemicon®
      TradeName
      • Chemicon
      DescriptionCaspase1,recombinanthumanactive
      OverviewCaspase-1(alsoknownasICE,Interleukin-1beta-ConvertingEnzyme)istheprototypicalmemberofthecaspasefamilyofcysteineproteases.Caspase-1existsincellsasaninactive45kDapro-enzyme.Thepro-enzymeismaturedbyproteolysistoyieldlarge(20kD)andsmall(10kD)subunits.Theactiveenzymeisaheterotetramerconsistingoftwolargeandtwosmallsubunits.Todatethemechanismofregulationofcaspase-1activationiscomplexandpoorlyunderstood.InTHP-1cells,alargeproportionofthecaspase-1ispresentintheinactivepro-enzymeform.

      Theactiverecombinanthumancaspase-1wasexpressedinE.coli.Theexpressedcaspase-1spontaneouslyundergoesautoprocessingtoyieldthesubunitscharacteristicofthenativeenzyme.Therecombinantcaspase-1preferentiallycleavesthesubstratesconsistingofconsensussequenceYVAD(e.g.,YVAD-AFCandYVAD-pNA).Therecombinantenzymecanalsobeusedasapositivecontrolincaspaseassaysorindeterminingthespecificityofcaspasesubstrates.

      Recommendedusage:0.5-1unitforfluorometriccaspaseassaysand2-4unitsforcolorimetriccaspaseassays.Optimalworkingdilutionsmustbedeterminedbyenduser.
      ProductInformation
      PresentationLyophilized.Reconstitutein25μLPBScontaining15%glycerol.
      StorageandShippingInformation
      StorageConditionsMaintainunopenedandrehydratedproductat-70°Cinundiluted,freeze-thawprotected,tightlysealedaliquots.Avoidrepeatedfreeze/thawcycles.Productstablefor6monthsfromdateofpurchasedifhandledappropriately.
      Applications
      KeyApplications
      • ActivityAssay
      BIOLOGicalInformation
      PurityPartiallypurifiedrecombinantcaspase3withafulllengthHIS-6-tag.Approximately10-20%caspase1protein.TheremainderisE.Coliproteins.Approximately0.8-2mgtotalprotein/Uofactivity;E.Coliproteinshavenoreactivity.
      EntrezGeneNumber
      EntrezGeneSummaryThisgeneencodesaproteinwhichisamemberofthecysteine-asparticacidprotease(caspase)family.Sequentialactivationofcaspasesplaysacentralroleintheexecution-phaseofcellapoptosis.Caspasesexistasinactiveproenzymeswhichundergoproteolyticprocessingatconservedasparticresiduestoproduce2subunits,largeandsmall,thatdimerizetoformtheactiveenzyme.ThisgenewasidentifiedbyitsABIlitytoproteolyticallycleaveandactivatetheinactiveprecursorofinterleukin-1,acytokineinvolvedintheprocessessuchasinflammation,septicshock,andwoundhealing.Thisgenehasbeenshowntoinducecellapoptosisandmayfunctioninvariousdevelopmentalstages.StudiesofasimilargeneinmousesuggestaroleinthepathogenesisofHuntingtondisease.Alternativesplicingofthisgeneresultsinfivetranscriptvariantsencodingdistinctisoforms.
      GeneSymbol
      • CASP1
      • IL1BCE
      • P45
      • IL-1BC
      • IL1BC
      • p45
      • IL1B-convertase
      • CASP-1
      • ICE
      • EC3.4.22.36[Contains:Caspase-1subunitp20
      • Caspase-1subunitp10].
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:P29466#ThiolproteasethatcleavesIL-1betabetweenanAspandanAla,releasingthematurecytokinewhichisinvolvedinavarietyofinflammatoryprocesses.Importantfordefenseagainstpathogens.Cleavesandactivatessterolregulatoryelementbindingproteins(SREBPs).Canalsopromoteapoptosis.
      SIZE:404aminoacids;45159Da
      SUBUNIT:Heterotetramerthatconsistsoftwoanti-parallelarrangedheterodimers,eachoneformedbya20kDa(p20)anda10kDa(p10)subunit.Thep20subunitcanalsoformaheterodimerwiththeepsilonisoformwhichthenhasaninhibitoryeffect.Maybeacomponentoftheinflammasome,aproteincomplexwhichalsoincludesPYCARD,CARD8andNALP2andwhosefunctionwouldbetheactivationofproinflammatorycaspases.
      SUBCELLULARLOCATION:Cytoplasm.
      TISSUESPECIFICITY:Expressedinlargeramountsinspleenandlung.Detectedinliver,heart,smallintestine,colon,thymus,prostate,skeletalmuscle,peripheralbloodleukocytes,kidneyandtestis.Noexpressioninthebrain.
      PTM:Thetwosubunitsarederivedfromtheprecursorsequencebyanautocatalyticmechanism.
      SIMILARITY:SwissProt:P29466##BelongstothepeptidaseC14family.&Contains1CARDdomain.
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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