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Millipore/CC123 | Caspase 8, recombinant human active/CC123/25 units
  • Millipore/CC123 | Caspase 8, recombinant human active/CC123/25 units

Millipore/CC123 | Caspase 8, recombinant human active/CC123/25 units

价格: ¥3204.00 市场价: 5340.00

货号: CC123
品牌: Millipore
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    • Description
      CatalogueNumberCC123
      BrandFamilyChemicon®
      TradeName
      • Chemicon
      DescriptionCaspase8,recombinanthumanactive
      OverviewTheactiverecombinanthumancaspase-8wasexpressedinE.coli.Theexpressedcaspase-8spontaneouslyundergoesautoprocessingtoyieldthesubunitscharacteristicoftheactiveenzyme.Therecombinantcaspase-8preferentiallycleavesthesubstratesconsistingofconsensussequenceIETD(e.g.,IETD-AFCandIETD-pNA).Therecombinantenzymecanalsobeusedasapositivecontrolincaspaseassaysorindeterminingthespecifictyofcaspasesubstrates.Recommendedusage:0.5-1unitforfluorometriccaspaseassaysand2-4unitsforcolorimetriccaspaseassays.Optimalworkingdilutionsmustbedeterminedbyenduser.
      AlternateNames
      • FLICE
      • MASH
      • Mch5
      BackgroundInformationCaspase-8isamemberoftheinterleukin-1βconvertingenzyme(ICE)familyofcysteineproteases.Aswithothercaspases,caspase-8alsoexistsincellsasaninactiveproenzyme.Duringapoptosisprocaspase-8isprocessedataspartateresiduesbyself-proteolysisand/orcleavagebyanothercaspase.Theprocessedformofcaspase-8consistsoflargeandsmallsubunitswhichassociatetoformtheactiveenzyme.Theactivecaspase-8hasbeenshowntobeinvolvedintheproteolysisofpoly(ADP-ribose)polymerase(PARP),anenzymethatisinvolvedinDNArepairandgenomicmaintenance.
      ProductInformation
      PresentationLyophilized.Reconstitutewith25μlPBScontaining15%glycerol.
      StorageandShippingInformation
      StorageConditionsMaintainat-70°Cfor3-6months.Avoidrepeatedfreeze/thawcycles.
      Applications
      KeyApplications
      • ActivityAssay
      ApplicationNotesRecommendedusage:0.5-1unitforfluorometriccaspaseassaysand2-4unitsforcolorimetriccaspaseassays.

      Optimalworkingdilutionsmustbedeterminedbyenduser.
      BIOLOGicalInformation
      SpecificActivity25U:*Whereoneunitoftherecombinantcaspase-8istheenzymeactivitythatcleaves1nmolofthecaspasesubstrateIETD-pNA(pNA:pnitroanaline)perhourat37°Cinareactionsolutioncontaining50mMHEPES,50mMNaCl,0.1%CHAPS,10mMEDTA,5%Glycerol,and10mMDTT.
      EntrezGeneNumber
      EntrezGeneSummaryThisgeneencodesamemberofthecysteine-asparticacidprotease(caspase)family.Sequentialactivationofcaspasesplaysacentralroleintheexecution-phaseofcellapoptosis.Caspasesexistasinactiveproenzymescomposedofaprodomain,alargeproteasesubunit,andasmallproteasesubunit.Activationofcaspasesrequiresproteolyticprocessingatconservedinternalasparticresiduestogenerateaheterodimericenzymeconsistingofthelargeandsmallsubunits.ThisproteinisinvolvedintheprogrammedcelldeathinducedbyFasandvariousapoptoticstimuli.TheN-terminalFADD-likedeatheffectordomainofthisproteinsuggeststhatitmayinteractwithFas-interactingproteinFADD.ThisproteinwasdetectedintheinsolublefractionoftheaffectedbrainregionfromHuntingtondiseasepatientsbutnotinthosefromnormalcontrols,whichimplicatedtheroleinneurodegenerativediseases.Manyalternativelysplicedtranscriptvariantsencodingdifferentisoformshavebeendescribed,althoughnotallvariantshavehadtheirfull-lengthsequencesdetermined.
      GeneSymbol
      • CASP8
      • MCH5
      • MGC78473
      • CASP-8
      • MACH
      • ALPS2B
      • FLICE
      • procaspase-8
      • CAP4
      • EC3.4.22.61[Contains:Caspase-8subunitp18
      • Caspase-8subunitp10].
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:Q14790#MostupstreamproteaseoftheactivationcascadeofcaspasesresponsIBLefortheTNFRSF6/FASmediatedandTNFRSF1Ainducedcelldeath.BindingtotheadaptermoleculeFADDrecruitsittoeitherreceptor.Theresultingaggregatecalleddeath-inducingsignalingcomplex(DISC)performsCASP8proteolyticactivation.TheactivedimericenzymeisthenliberatedfromtheDISCandfreetoactivatedownstreamapoptoticproteases.ProteolyticfragmentsoftheN-terminalpropeptide(termedCAP3,CAP5andCAP6)arelikelyretainedintheDISC.CleavesandactivatesCASP3,CASP4,CASP6,CASP7,CASP9andCASP10.MayparticipateintheGZMBapoptoticpathways.CleavesADPRT.Hydrolyzesthesmall-moleculesubstrate,Ac-Asp-Glu-Val-Asp--AMC.LikelytargetforthecowpoxvirusCRMAdeathinhibitoryprotein.Isoforms5,6,7and8lackthecatalyticsiteandmayinterferewiththepro-apoptoticactivityofthecomplex.
      SIZE:479aminoacids;55391Da
      SUBUNIT:Heterotetramerthatconsistsoftwoanti-parallelarrangedheterodimers,eachoneformedbya18kDa(p18)anda10kDa(p10)subunit.InteractswithFADD,CFLARandPEA15.Isoform9interactsattheendoplasmicreticulumwithacomplexcontainingBCAP31,BAP29,BCL2and/orBCL2L1.
      SUBCELLULARLOCATION:Cytoplasm.
      TISSUESPECIFICITY:Isoforms1,5and7areexpressedinawidevarietyoftissues.Highestexpressioninperipheralbloodleukocytes,spleen,thymus,andliver.Barelydetectableinbrain,testis,andskeletalmuscle.DOMAIN:SwissProt:Q14790Isoform9containsaN-terminalextensionthatisrequiredforinteractionwiththeBCAP31complex.
      PTM:Generationofthesubunitsrequiresassociationwiththedeath-inducingsignalingcomplex(DISC),whereasadditionalprocessingislikelyduetotheautocatalyticactivityoftheactivatedprotease.GZMBandCASP10canbeinvolvedintheseprocessingevents.&PhosphorylateduponDNAdamage,probablybyATMorATR.
      DISEASE:SwissProt:Q14790#DefectsinCASP8arethecauseofcaspase-8deficiency(CASP8D)[MIM:607271].CASP8Disadisorderresemblingautoimmunelymphoproliferativesyndrome(ALPS).Itischaracterizedbylymphadenopathy,splenomegaly,anddefectiveCD95-inducedapoptosisofperipheralbloodlymphocytes(PBLs).ItleadstodefectsinactivationofT-lymphocytes,B-lymphocytes,andnaturalkillercellsleADIngtoimmunodeficiencycharacterizedbyrecurrentsinopulmonaryandherpessimplexvirusinfectionsandpoorresponsestoimmunization.
      SIMILARITY:BelongstothepeptidaseC14family.&Contains2DED(deatheffector)domains.
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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