HumanCD4+TCell.ImagecourtesyofNIAID.
Tcellsareregulatorsoftheimmunesystem.CD4+TcellsarealsoknownashelperTcellsbecausetheyactonothercellsoftheimmunesystemtopromotevariousaspectsoftheimmuneresponse,includingimmunoglobulinisotypeswitchingandaffinitymaturationoftheantibodyresponse,macrophageactivationandenhancedactivityofnaturalkillercellsandcytotoxicTcells.
CellsYouCanCountontoPerform
LonzaCD4+TCellsareisolatedfromperipheralbloodofhealthydonors.Briefly,mononuclearcells(MNCsorPBMCs)areisolatedfromapheresisderivedperipheralbloodandarefurtherpurifiedviaimmunomagneticselectiondirectedagainstCD4.
CryopreservedCD4+Tcellsareguaranteed≥90%pureforCD4+Tcells,asassessedbyflowcytometry.Theyareguaranteedtocontain≥10millionviablecellspervial.HIV-1,hepatitisBandhepatitisCarenotdetectedforalldonorsand/orcelllots.ACertificateofAnalysisisprovidedforeachcelllotpurchased.
Cellsareavailablefrommultipledonors.PleasecontactCustomerServicefordetailsonavailableinventoryanddonordemographiccharacteristics(age,gender,andethnicity).
WhitePaper:Th17CellDifferentiation Th17cellsarestartingtocomeintothespotlightforpotentialimmunotherapytreatments.OneofthemajorchallengesinstudyingTh17cellularactionsisobtainingenoughcellsforanygivenexperiment.ReadthiswhitepapertolearnaboutarobustmethodtodifferentiateperipheralbloodCD4+TcellsintofunctionalTh17cellsusingabead-basedactivationtechnology. | DownloadWhitepaper |
ChoicesinCellTypeandTissueSource
Hematopoieticandimmuneresearchrequirescellsfromdifferentdonorsandtissuesources.That"swhyLonzaoffersmanydifferentperipheralbloodcelltypes.Ifyouneeddifferentcelltypesfromthesamedonor,youcaneitherisolatefromLonzacryopreservedPBMCsorcontactCustomerServicetoinquireaboutcellsmatchedfromthesamedonor.
Inaddition,ifyouareinterestedinmorenaiveTcells,unprocessedbonemarrow,autologousbonemarrowandwholebloodfromthesamedonor,bonemarrowCD34+cells,orcordbloodCD34+cellsmightbegoodsolutions.
SupportedbyLonzaMedia
LGM-3™LymphocyteGrowthMediumisrecommendedforoptimalperformance.PleasecontactScientificSupportforthawingandculturingguidance.
LonzaguaranteestheperformanceofClonetics™/Poietics™cellsonlyifappropriateClonetics™/Poietics™mediaandreagentsareusedexclusivelyandtherecommendedstorageanduseprotocolsarefollowed.Anymodificationsmadetotherecommendedcellsystemsincludingtheuseofalternativemedia,reagentsorprotocols,willvoidcellandmediaperformanceguarantees.Ifyouneedassistanceinselectingtheappropriatemedia,reagents,orprotocol,pleasecontactLonzaScientificSupport.