当前位置 : Millipore >>> Millipore/17-10047 | ChIPAb+ Histone H4 - ChIP Validated Antibody and Primer Set, rabbit monoclonal/17-10047/25 assays25
Millipore/17-10047 | ChIPAb+ Histone H4 - ChIP Validated Antibody and Primer Set, rabbit monoclonal/17-10047/25 assays25
  • Millipore/17-10047 | ChIPAb+ Histone H4 - ChIP Validated Antibody and Primer Set, rabbit monoclonal/17-10047/25 assays25

Millipore/17-10047 | ChIPAb+ Histone H4 - ChIP Validated Antibody and Primer Set, rabbit monoclonal/17-10047/25 assays25

价格: 试用 市场价: 0.00

货号: 17-10047
品牌: Millipore
规格
数量
库存(0)
特别 提示
代购产品:无质量问题不接受退换货,下单前请仔细核对信息。
下单后请及时联系客服核对商品价格,订单生效后再付款。
资深产品顾问
咨询顾问

全国免费服务热线

4000-520-616


  • 自营商城 一站式服务
  • 厂家直采 剔除溢价
  • 品质甄选 正品保证
  • 严控流程 只做188精品
  • 极速物流 如约送货
  • 详情
  • 使用说明
  • 常见问题
    • Description
      CatalogueNumber17-10047
      BrandFamilyUpstate
      TradeName
      • ChIPAb+
      • Upstate
      DescriptionChIPAb+HistoneH4-ChIPValidatedAntibodyandPrimerSet,rabbitmonoclonal
      OverviewAllChIPAb+antibodiesareindividuallyvalidatedforchromatinprecipitation,everylot,everytime.EachChIPAb+antibodysetincludescontrolprimers(testedeverylotbyqPCR)toBIOLOGicallyvalidateyourIPresultsinalocus-specificcontext.TheqPCRprotocolandprimersequencesareprovided,allowingresearcherstovalidateChIPprotocolswhenusingourantibodyintheirchromatincontext.EachsetalsoincludesanegativecontrolantibodytoensurespecificityoftheChIPreaction.
      TheChIPAb+HistoneH4setincludestheHistoneH4antibody,anegativecontrolantibody(normalrabbitIgG),andqPCRprimerswhichamplifya110bpregionofhumanβ-Globin.TheHistoneH4andnegativecontrolantibodiesaresuppliedinascalable"perChIP"reactionsizeandcanbeusedtofunctionallyvalidatetheprecipitationofHistoneH4-associatedchromatin.
      AlternateNames
      • H4
      • HistoneH4
      • H4histonefamily,memberA
      • histone1,H4a
      • histonecluster1,H4a
      BackgroundInformationHistoneH4isoneofthe5mainhistoneproteinsinvolvedinthestructureofchromatinineukaryoticcells.FeaturingamainglobulardomainandalongNterminaltailH4isinvolvedwiththestructureofthenucleosomesofthe"beadsonastring"structure.
      ProductInformation
      FormatPurified
      Control
      • Includesnegativecontrolantibody(normalrabbitIgG)andprimersspecificforhumanβ-Globin.
      PresentationAnti-HistoneH4(rabbitmonoclonalIgG).Onevialcontaining50µLofproteinApurifiedrabbitmonoclonalIgGsupernatantinbuffercontaining0.1MTris-Glycine(pH7.4),150mMNaClwith0.05%sodiumazidebeforeadditionofglycerolto40%.Storeat-20°C.

      NormalRabbitIgG.Onevialcontaining75µgofnormalrabbitIgGin75µLstoragebuffer.Storeat-20°C.

      ChIPPrimers,β-Globin.Onevialcontaining75μLof5μMofeachprimerspecificforhumanβ-Globin.Storeat-20°C.
      FOR:AGGACAGGTACGGCTGTCATC
      REV:TTTATGCCCAGCCCTGGCTC

      StorageandShippingInformation
      StorageConditionsStablefor1yearat-20°Cfromdateofreceipt.HandlingRecommendations:Uponfirstthaw,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance.Note:VariABIlityinfreezertemperaturesbelow-20°Cmaycauseglycerolcontainingsolutionstobecomefrozenduringstorage.
      Applications
      ApplicationThisChIPAb+HistoneH4-ChIPValidatedAntibody&PrimerSetconvenientlyincludestheantibody&thespecificcontrolPCRprimers.
      KeyApplications
      • WesternBlotting
      • ChromatinImmunoprecipitation(ChIP)
      ApplicationNotesChromatinImmunoprecipitation:
      SonicatedchromatinpreparedfromHeLacells(1X106cellequivalentsperIP)weresubjectedtochromatinimmunoprecipitationusing2µLofeithernormalrabbitIgGor2µLofAnti-HistoneH4antibodyandtheMagnaChIP™AKit(Cat.#17-610).
      SuccessfulimmunoprecipitationoftotalHistoneH4associatedDNAfragmentswasverifiedbyqPCRusingChIPPrimers,β-Globin,aswellasGAPDHpromoterControlPrimers,(Pleaseseefigures).
      DataispresentedaspercentinputofeachIPsamplerelativetoinputchromatinforeachampliconandChIPsampleasindicated.

      PleaserefertotheEZ-MagnaChIP™A(Cat.#17-408)orEZ-ChIP™(Cat.#17-371)protocolforexperimentaldetails.

      WesternBlotAnalysis:
      RepresentativeLotData.
      HeLaAcidExtract(Lane1and3),HeLaAcidExtractfromsodiumbutyratetreatedcells(Lane2),HeLaAcidExtractfromcolcemidtreatedcells(Lane4)andrecombinanthistoneH4(Lane5)wereresolvedbyelectrophoresis,transferredtoPVDFmembranesandprobedwithAnti-HistoneH4(1:1,000dilution).
      ProteinswerevisualizedusingaDonkeyanti-rabbitIgGconjugatedtoHRPandvisualizedusingachemiluminescencedetectionsystem.(Pleaseseefigures).
      BiologicalInformation
      ImmunogenKLH-conjugatedsyntheticpeptidecorres-pondingtoaminoacids17-28ofHistoneH4.
      Epitopea.a25-28
      HostRabbit
      SpecificityRecognizesHistoneH4.
      SpeciesReactivity
      • Human
      SpeciesReactivityNoteHuman.Widerangeofcross-reactivityexpectedbasedonsequencehomology.
      AntibodyTypeMonoclonalAntibody
      EntrezGeneNumber
      EntrezGeneSummaryHistonesarebasicnuclearproteinsthatareresponsIBLeforthenucleosomestructureofthechromosomalfiberineukaryotes.Thisstructureconsistsofapproximately146bpofDNAwrappedaroundanucleosome,anoctamercomposedofpairsofeachofthefourcorehistones(H2A,H2B,H3,andH4).Thechromatinfiberisfurthercompactedthroughtheinteractionofalinkerhistone,H1,withtheDNAbetweenthenucleosomestoformhigherorderchromatinstructures.ThisgeneisintronlessandencodesamemberofthehistoneH4family.TranscriptsfromthisgenelackpolyAtails;instead,theycontainapalindromicterminationelement.Thisgeneisfoundinahistoneclusteronchromosome1.Thisgeneisoneoffourhistonegenesintheclusterthatareduplicated;thisrecordrepresentsthetelomericcopy.[providedbyRefSeq].
      GeneSymbol
      • HIST1H4A
      • H4/J
      • HIST1H4F
      • HIST1H4L
      • H4FN
      • H4FA
      • H4FH
      • HIST1H4H
      • H4F2
      • H4FM
      • H4/A
      • H4FK
      • H4FG
      • HIST2H4
      • H4FB
      • H4/K
      • HIST1H4I
      • H4/N
      • HIST1H4B
      • H4FE
      • H4/M
      • H4/a
      • HIST1H4E
      • HIST4H4
      • HIST2H4A
      • H4FC
      • H4FI
      • H4/H
      • HIST1H4C
      • H4/G
      • HIST1H4K
      • H4FJ
      • H4FD
      • H4/I
      • H4/B
      • H4/D
      • H4/C
      • HIST1H4J
      • HIST1H4D
      • H4/E
      PurificationMethodProteinAPurfied
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:P62805#Corecomponentofnucleosome.NucleosomeswrapandcompactDNAintochromatin,limitingDNAaccessibilitytothecellularmachinerieswhichrequireDNAasatemplate.Histonestherebyplayacentralroleintranscriptionregulation,DNArepair,DNAreplicationandchromosomalstability.DNAaccessibilityisregulatedviaacomplexsetofpost-translationalmodificationsofhistones,alsocalledhistonecode,andnucleosomeremodeling.
      SIZE:103aminoacids;11367Da
      SUBUNIT:ThenucleosomeisahistoneoctamercontainingtwomoleculeseachofH2A,H2B,H3andH4assembledinoneH3-H4heterotetramerandtwoH2A-H2Bheterodimers.Theoctamerwrapsapproximately147bpofDNA.
      SUBCELLULARLOCATION:Nucleus.
      PTM:SymmetricdimethylationonArg-4bythePRDM1/PRMT5complexmayplayacrucialroleinthegerm-celllineage(Bysimilarity).&UbiquitinatedbytheCUL4-DDB-RBX1complexinresponsetoultravioletirrADIation.ThismayweakentheinteractionbetweenhistonesandDNAandfacilitateDNAaccessibilitytorepairproteins.
      SIMILARITY:SwissProt:P62805##BelongstothehistoneH4family.
      MolecularWeight~11kDa
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
    售后保障
    蚂蚁淘生物188,秉承蚂蚁淘一贯的严谨态度,由蚂蚁淘公司专业人员负责品控、采购、物流、销售、售后,保障正品优质。以“快速好省,为科研提供好产品、好价格”为理念,直接链接原厂家,从全国各地原制造商严格挑选188款科研精品,剔除品牌溢价,188生物新电商,把好的产品带给科研!  力求给你最优质的商品。
  • Q:生物188产品正品保障吗?
    A:生物188质量把控人员具有十年的从业经验,在业界享有良好的口碑;自营商城,直接从厂家采购, 自己的团队负责国际物流和清关,中间没有第三方,所有流程严格把控,100%保证正品,假一罚十。

    Q:下单后可以修改订单吗?
    A:下单后的商品付款之前可以修改;订单付款成功,需要联系我们客服进行修改;客服电话:4000-520-616

    Q:可以开发票吗?
    A:本网站所售商品都是正规清关,均开具16%正规发票,发票金额含配送费金额,另有说明的除外。

    Q:商品几天可以发货?
    A:生物188商品,全部现货销售,付款后即可发货,一般一周内送达!

    Q:如何联系商家?
    A:有任何问题够可以电话咨询我们,全国24小时免费服务热线:4000-520-616 或联系我们的在线客服QQ:1570468124

    Q:收到的商品少了/发错了怎么办?
    A:同个订单购买多个商品可能会分为一个以上包裹发出,可能不会同时送达,建议查看订单详情是否是 部分发货状态;如未收到,可联系在线客服或者致电4000-520-616。

    Q:退换货/维修需要多长时间?
    A:一般情况下,退货处理周期为客户收到产品一个月内(以快递公司显示签收时间为准),包装规格、 数量、品种不符,外观毁损、短缺或缺陷,请在收到货24小时内申请退换货;特殊商品以合同条款为准。

何为188

极简而严谨,我们仅销售188款生物医学科研用品,款款都是爆款;因为少所以聚焦,聚焦甄选每一款产品,聚焦服务每一位客户!

关注我们 :

点击QQ联系我们
生物188微信

关注188微信公众号

获取最新优惠活动通知
  • 品质甄选,正品保证

  • 自营电商,厂家直采

  • 极简主义,188精品