AdditionalDonorInformation:
Description:
M1peptideisasyntheticpeptiderepresentsresidues58-66oftheinfluenzaAmatrixprotein.InHLA-A*0201subjectstheCD8+CTLresponseisdominatedbycellsexpressingVβ17andrespondingtothispeptide.
Structure:ThesequenceofthepeptideisGILGFVFTL
Storage:Storefrozenat≤minus20oC
Activity:
Theoptimumconcentrationdependsonthespecificuseofthepeptide.TostimulatePBMC,concentrationsof1-10ug/mLareappropriate.SpecificTcellclonesandlinesrespondwelltomuchlowerconcentrations.
References
MossPA,MootsRJ,RosenbergWM,etal.(1991)ExtensiveconservationofalphaandbetachainsofthehumanT-cellantigenreceptorrecognizingHLA-A2andinfluenzaAmatrixpeptide.Proc.NatlAcad.Sci.88:8987.
LehnerPJ,WangEC,MossPA,etal.(1995)HumanHLA-A0201-restrictedcytotoxicTlymphocyterecognitionofinfluenzaisdominatedbyTcellsbearingtheVbeta17genesegment.JExpMed.181:79