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Millipore/07-821 | Anti-phospho-EGFR (Tyr992) Antibody/07-821/100 µL
  • Millipore/07-821 | Anti-phospho-EGFR (Tyr992) Antibody/07-821/100 µL

Millipore/07-821 | Anti-phospho-EGFR (Tyr992) Antibody/07-821/100 µL

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货号: 07-821
品牌: Millipore
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    • Description
      Catalogue Number07-821
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-phospho-EGFR (Tyr992) Antibody
      Alternate Names
      • epidermal growth factor receptor
      • ERBB
      • ERBB1
      • mENA
      Product Information
      FormatAffinity Purified
      Presentation100 µL of affinity purified rabbit polyclonal IgG in 50% storage buffer (PBS (without Mg2+ and Ca2+), pH 7.3 containing 1.0 mg/mL BSA (IgG, protease free) and 0.05% sodium azide) and 50% glycerol.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C from date of shipment
      Applications
      ApplicationAnti-phospho-EGFR (Tyr992) Antibody detects level of phospho-EGFR (Tyr992) & has been published & validated for use in WB.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenPeptide corresponding to amino acid region encompassing the human, mouse, and rat phospho-EGFR (Tyr992).
      HostRabbit
      SpecificityRecognizes phospho-EGFR (Tyr992)
      IsotypeIgG
      Species Reactivity
      • Human
      Species Reactivity NotePredicted to cross-react with mouse and rat based on sequence homology, but have not been tested.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • EGFR
      • ERBB1
      • ERBB
      • mENA
      Modifications
      • Phosphorylation
      Purification MethodImmunoAffinity Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P00533 # Isoform 2/truncated isoform may act as an antagonist.
      SIZE: 1210 amino acids; 134277 Da
      SUBUNIT: Binds RIPK1. CBL interacts with the autophosphorylated C- terminal tail of the EGF receptor. Part of a complex with ERBB2 and either PIK3C2A or PIK3C2B. The autophosphorylated form interacts with PIK3C2B, maybe indirectly. Interacts with PELP1.
      SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. & Isoform 2: Secreted.
      TISSUE SPECIFICITY: Expressed in placenta. Isoform 2 is also expressed in ovarian cancers.
      PTM: Phosphorylation of Ser-695 is partial and occurs only if Thr- 693 is phosphorylated. & Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occur.DISEASE:SwissProt: P00533 # Defects in EGFR are associated with lung cancer [MIM:211980].
      SIMILARITY: SwissProt: P00533 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily. & Contains 1 protein kinase domain.
      MISCELLANEOUS: Binding of EGF to the receptor leads to dimerization, internalization of the EGF-receptor complex, induction of the tyrosine kinase activity, stimulation of cell DNA synthesis, and cell proliferation.
      Molecular Weight185 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Materials Information
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