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Millipore/07-329 | Anti-acetyl-Histone H4 (Lys16) Antibody/07-329/100 µL
  • Millipore/07-329 | Anti-acetyl-Histone H4 (Lys16) Antibody/07-329/100 µL

Millipore/07-329 | Anti-acetyl-Histone H4 (Lys16) Antibody/07-329/100 µL

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货号: 07-329
品牌: Millipore
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    • Description
      CatalogueNumber07-329
      BrandFamilyUpstate
      TradeName
      • Upstate
      DescriptionAnti-acetyl-HistoneH4(Lys16)Antibody
      AlternateNames
      • H4K16Ac
      • HistoneH4(acetylK16)
      • H4histonefamily,memberA
      • histone1
      • H4a
      • histonecluster1
      • H4a
      BackgroundInformationHistoneH4isoneofthe5mainhistoneproteinsinvolvedinthestructureofchromatinineukaryoticcells.FeaturingamainglobulardomainandalongNterminaltailH4isinvolvedwiththestructureofthenucleosomesofthe"beadsonastring"structure.AcetylationofhistoneH4occursatseveraldifferentlysinepositionsinthehistonetailandisperformedbyafamilyofenzymesknownasHistoneAcetylTransferases(HATs).
      ProductInformation
      FormatAffinityPurified
      Control
      • TSAtreatedNIH/3T3cells.
      • HeLaacidextract
      PresentationPurifiedrabbitpolyclonalinbuffercontaining0.1MTris-Glycine(pH7.4)150mMNaClwith0.05%sodiumazide.
      StorageandShippingInformation
      StorageConditionsStablefor1yearat2-8°Cfromdateofreceipt.
      Formaximumrecoveryofproduct,centrifugethevialpriortoremovingthecap.
      Applications
      ApplicationAnti-acetyl-HistoneH4(Lys16)Antibodyisarabbitpolyclonalantibodyfordetectionofacetyl-HistoneH4(Lys16)alsoknownasH4K16Ac,HistoneH4(acetylK16&hasbeenpublishedandvalidatedinChIP,WB,Mplex,PIA,DB,ChIP-seq.
      KeyApplications
      • WesternBlotting
      • Multiplexing
      • PeptideInhibitionAssay
      • DotBlot
      • ChIP-seq
      • ChromatinImmunoprecipitation(ChIP)
      ApplicationNotesImmunocytochemistryAnalysis:A1:500dilutionfromarepresentativelotdetectedAcetyl-HistoneH4(Lys16)inHeLa,A431andNIH/3T3cells.
      ChromatinImmunoprecipitation(ChIP)Analysis:1µgfromarepresentativelotimmunoprecipitatedAcetyl-HistoneH4(Lys16)in1µLofHeLacelllysate.
      DotBlotAnalysis:A1:5,000dilutionfromarepresentativelotdetectedAcetyl-HistoneH4(Lys16)inanAbsurance™HistoneH3AntibodySpecificityArray(Cat.No.16-667)andanAbsurance™HistoneH2A,H2B,H4AntibodySpecificityArray(Cat.No.16-665).
      Multiplexing(MPLX):ThisantibodyspecificallyrecognizeshistoneH4acetylatedonLys16byLuminex®assay.
      PeptideInhibitionAssay(PIA):ThisantibodypeptideblockedonHeLacellextracts.
      ChIP-SeqAnalysis:Arepresentativelotofthisantibodywasusedbytwoindependent
      laboratoriesforChIP-Seq(StromeandAhringerLaboratory,UCSantaCruz/Universityof
      CambridgeandMitziKurodaLaboratory,HarvardMedicalSchool)SeeEgelhofer,T.A.,etal.(2011).
      BIOLOGicalInformation
      ImmunogenOvalbumin-conjugated,syntheticpeptide(…GAAcKRH…)inwhichAcKcorrespondstoresidue16ofyeasthistoneH4.
      EpitopeLys16
      ConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
      HostRabbit
      IsotypeIgG
      SpeciesReactivity
      • Human
      • Mouse
      • Rat
      SpeciesReactivityNoteBroadspeciescross-reactivityisexpected.
      AntibodyTypePolyclonalAntibody
      EntrezGeneNumber
      GeneSymbol
      • HIST1H4A
      • H4/J
      • HIST1H4F
      • HIST1H4L
      • H4FN
      • H4FA
      • H4FH
      • HIST1H4H
      • H4F2
      • H4FM
      • H4/A
      • H4FK
      • H4FG
      • HIST2H4
      • H4FB
      • H4/K
      • HIST1H4I
      • H4/N
      • HIST1H4B
      • H4FE
      • H4/M
      • H4/a
      • HIST1H4E
      • HIST4H4
      • HIST2H4A
      • H4FC
      • H4FI
      • H4/H
      • HIST1H4C
      • H4/G
      • HIST1H4K
      • H4FJ
      • H4FD
      • H4/I
      • H4/B
      • H4/D
      • H4/C
      • HIST1H4J
      • HIST1H4D
      • H4/E
      Modifications
      • Acetylation
      PurificationMethodAffinityPurfied
      UniProtNumber
      MolecularWeight10kDa
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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