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Millipore/05-1004 | Anti-phospho-EGFR (Tyr1173) Antibody, clone 3G3.2/05-1004/100 µg
  • Millipore/05-1004 | Anti-phospho-EGFR (Tyr1173) Antibody, clone 3G3.2/05-1004/100 µg

Millipore/05-1004 | Anti-phospho-EGFR (Tyr1173) Antibody, clone 3G3.2/05-1004/100 µg

价格: ¥3576.00 市场价: 5960.00

货号: 05-1004
品牌: Millipore
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    • Description
      CatalogueNumber05-1004
      DescriptionAnti-phospho-EGFR(Tyr1173)Antibody,clone3G3.2
      AlternateNames
      • C-typelectindomainfamily14,memberA
      • ClECTandEGF-likedomaincontainingprotein
      • Epidermalgrowthfactorreceptor5
      • chromosome14openreADIngframe27
      BackgroundInformationTheepidermalgrowthfactorreceptor(EGFR;ErbB-1;HER1inhumans)isthecell-surfacereceptorformembersoftheepidermalgrowthfactorfamily(EGF-family)ofextracellularproteinligands.TheepidermalgrowthfactorreceptorisamemberoftheErbBfamilyofreceptors,asubfamilyoffourcloselyrelatedreceptortyrosinekinases:EGFR(ErbB-1),HER2/c-neu(ErbB-2),Her3(ErbB-3)andHer4(ErbB-4).MutationsaffectingEGFRexpressionoractivitycouldresultincancer.EGFR(epidermalgrowthfactorreceptor)existsonthecellsurfaceandisactivatedbybindingofitsspecificligands,includingepidermalgrowthfactorandtransforminggrowthfactorα(TGFα).EGFRdimerizationstimulatesitsintrinsicintracellularprotein-tyrosinekinaseactivity.Asaresult,autophosphorylationoffivetyrosine(Y)residuesintheC-terminaldomainofEGFRoccurs.
      ProductInformation
      FormatPurified
      Control
      • EGFtreatedA431celllysate
      PresentationPurifiedmouseascitescontaining0.1MTris-glycine,pH7.4,150mMNaCland0.05%sodiumazide.
      StorageandShippingInformation
      StorageConditionsMaintainrefrigeratedat2-8°Cfor1yearfromdateofreceipt.
      Applications
      ApplicationDetectphospho-EGFR(Tyr1173)usingthisAnti-phospho-EGFR(Tyr1173)Antibody,clone3G3.2validatedforuseinWB,FC,IH&IC.
      KeyApplications
      • WesternBlotting
      • FlowCytometry
      • Immunohistochemistry
      • Immunocytochemistry
      ApplicationNotesFlowCytometryAnalysis:A431cellsstainedusing10ug/mLofanti-phospho-EGFR(Tyr1173).
      ImmunocytochemistryAnalysis:A1:500dilutionofthisantibody
      detectedanti-phospho-EGFR(Tyr1173)inEGFtreatedA431
      lysates.
      ImmunohistochemistryAnalysis:A1:100dilutionofthisdetectedanti-phospho-EGFR(Tyr1173)inSEROusovariancancer.
      BIOLOGicalInformation
      ImmunogenKLH-conjugatedlinearpeptidecorrespondingtoresiduessurroundingTyr1173ofEGFR.
      EpitopePhosphorylatedTyr1173
      Clone3G3.2
      ConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
      HostMouse
      SpecificityThisantibodyrecognizesphosphorylatedEGFR(Tyr1173).
      IsotypeIgG1κ
      SpeciesReactivity
      • Human
      SpeciesReactivityNoteProventoreactwithhuman.
      AntibodyTypeMonoclonalAntibody
      EntrezGeneNumber
      EntrezGeneSummaryTheproteinencodedbythisgeneisatransmembraneglycoproteinthatisamemberoftheproteinkinasesuperfamily.Thisproteinisareceptorformembersoftheepidermalgrowthfactorfamily.EGFRisacellsurfaceproteinthatbindstoepidermalgrowthfactor.Bindingoftheproteintoaligandinducesreceptordimerizationandtyrosineautophosphorylationandleadstocellproliferation.Mutationsinthisgeneareassociatedwithlungcancer.
      GeneSymbol
      • C14orf27
      • CEG1
      • EGFR-5
      • EGFR5
      • OTTHUMP00000178873
      Modifications
      • Phosphorylation
      PurificationMethodProteinGPurified
      UniProtNumber
      UniProtSummarySUBCELLULARLOCATION:Membrane;Single-passtypeImembraneproteinPotential.
      SEQUENCESIMILARITIES:Contains1C-typelectindomain.
      Contains1EGF-likedomain.
      MolecularWeight175kDa
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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