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Millipore/01-185 | VEGF Protein, Human recombinant/01-185/10 µg
  • Millipore/01-185 | VEGF Protein, Human recombinant/01-185/10 µg

Millipore/01-185 | VEGF Protein, Human recombinant/01-185/10 µg

价格: ¥4164.00 市场价: 6940.00

货号: 01-185
品牌: Millipore
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    • Description
      CatalogueNumber01-185
      BrandFamilyUpstate
      TradeName
      • Upstate
      DescriptionVEGFProtein,Humanrecombinant
      ProductInformation
      Formulation
      Presentation10µgofVEGFlyophilizedproteinfrombuffercontaining25mMHEPESand150mMNaCl,pH7.0.Carrierfree.
      StorageandShippingInformation
      StorageConditionsThelyophilizedpowderisstableforafewweeksatroomtemperature,butbeststoredat-20°Cforuptooneyearfromdateofreceipt.Afteraquickspin,reconstitutewithsterilewateror25mMHEPESand150mMNaCl,pH7.0.Reconstitutedmaterialcanbestoredat2°-8°Cforoneweek,butundilutedaliquotsat-20°Carerecommended.Avoidrepeatedfreeze/thawcycles.

      Thisproductisacidstable.Diluteineither0.1%TFAor4mMHCl,containing25ugBSAperugVEGFtomaintainstABIlity.Reducingreagents(suchasdithiothrietolor2-mercaptoethanol)inhibitVEGFactivity.
      Applications
      ApplicationThehumanrecombinantVEGFProteinisavailableina10µgformat.
      KeyApplications
      • CellCulture
      ApplicationNotesVEGFProliferationAssay

      1.Priortouseinthisassay,maintainHUVEcellsinF-12Kmediumsupplementedwith10%(v/v)FBS,1%penicillin-streptomycin-glutamine,0.1mg/mlheparinand0.05mg/mlendothelialcellgrowthsupplement(ECGS).

      2.ForassayofVEGF,subcultureHUVEcellsintoa96-wellplateatadensityof4,000cellsperwellinthegrowthmediumovernight.

      3.AddVEGFtoafinalconcentrationrangeof0.2,0.4,0.8,1.6,3.2,6.2,12.5,25and50ng/ml.AddVEGFdiluenttoonesetofwellsasthenegativecontrol.
      4.Fourdayslater,add20ulofPromegaSubstrateCellTiter96AqueousOneSolutionReagenttoeachwell.
      5.Incubateat37Cfor2hrsandreadatOD490nm.
      BIOLOGicalInformation
      SourceRecombinanthumanDNAexpressedinbaculovirusinfectedSf9cells
      EntrezGeneNumber
      EntrezGeneSummaryThisgeneisamemberofthePDGF/VEGFgrowthfactorfamilyandencodesaproteinthatisoftenfoundasadisulfidelinkedhomodimer.Thisproteinisaglycosylatedmitogenthatspecificallyactsonendothelialcellsandhasvariouseffects,includingmediatingincreasedvascularpermeability,inducingangiogenesis,vasculogenesisandendothelialcellgrowth,promotingcellmigration,andinhibitingapoptosis.ElevatedlevelsofthisproteinislinkedtoPOEMSsyndrome,alsoknownasCrow-Fukasesyndrome.Mutationsinthisgenehavebeenassociatedwithproliferativeandnonproliferativediabeticretinopathy.Alternativelysplicedtranscriptvariants,encodingeitherfreelysecretedorcell-associatedisoforms,havebeencharacterized.Thereisalsoevidencefortheuseofnon-AUG(CUG)translationinitiationsitesupstreamof,andin-framewiththefirstAUG,leADIngtoadditionalisoforms.
      GeneSymbol
      • VEGFA
      • VEGF-A
      • VEGF
      • VPF
      • MGC70609
      PurificationMethodSterilizedthrougha0.2micronmembranefilter
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:P15692#Growthfactoractiveinangiogenesis,vasculogenesisandendothelialcellgrowth.Inducesendothelialcellproliferation,promotescellmigration,inhibitsapoptosis,andinducespermeabilizationofbloodvessels.BindstotheVEGFR1/Flt-1andVEGFR2/Kdrreceptors,heparansulfateandheparin.Neuropilin-1bindsisoformsVEGF-165andVEGF-145.
      SIZE:232aminoacids;27042Da
      SUBUNIT:Homodimer;disulfide-linked.AlsofoundasheterodimerwithPlGF(Bysimilarity).
      SUBCELLULARLOCATION:Secreted.Note=VEGF121isacidicandfreelysecreted.VEGF165ismorebasic,hasheparin-bindingpropertiesand,althoughasignicantproportionremainscell-associated,mostisfreelysecreted.VEGF189isverybasic,itiscell-associatedaftersecretionandisboundavidlybyheparinandtheextracellularmatrix,althoughitmaybereleasedasasolubleformbyheparin,heparinaseorplasmin.
      TISSUESPECIFICITY:TheVEGF189,VEGF-165andVEGF-121isoformsarewidelyexpressed,whereastheVEGF206andVEGF-145areuncommon.
      SIMILARITY:SwissProt:P15692##BelongstothePDGF/VEGFgrowthfactorfamily.
      StemCellType
      • NeuralStemCells
      • MesenchymalStemCells
      • HematopoieticStemCells
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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