ProductOverview:
Anewrobustandversatileserum-freemediumformulationforhighandlowdensityNHEK-NormalHumanEpidermalKeratinocytecultures
KGM-Gold™KeratinocyteGrowthMediumisdesignedtosupportbothclonalgrowthandhighdensitykeratinocyteproliferation.ThemediumsupportssuperiorgrowthandmorphologyofkeratinocytesisolatedinKGM-2™(datanotshown),KGM™(datanotshown)andCompetitorA"sMedium(datashownbelow).
FurThermore,qualitycontrolatLonzarequiresthatkeratinocytespassstrictmorphologicalcriteriaspecifyingtheminimalpresenceofenlargedandvacuolatedcells,enablingLonzakeratinocytestobeusedbytheresearcherfor≥15PopulationDoublings.
Adultandneonatalkeratinocytesarealsoavailable.
RecommendedSubculturingReagents: ReagentPackTMSubcultureReagents
Inaside-by-sidecomparisonwithamajorcompetitor’sformulation,culturesmaintainedinKGM-Gold™KeratinocyteGrowthMediumhadamorehomogeneousphenotypeconsistingofsmallrefractilecellswithminimalenlargementandvacuolization.TheABIlityofKGM-Gold™MediumtopromotethehealthofNHEK-NormalHumanEpidermalKeratinocytecellspositivelyinfluenceskeratinocyteLifespan.
Figure4A: GrowthofLonza’sadultkeratinocytesgrowninKGM-Gold™GrowthMediumcomparedtoCompetitorA’sadultkeratinocytesintheirmedium.
| Lonza’sadultkeratinocytesgrowninKGM-Gold™outperformedcompetitorA’sadultkeratinocytesgrownintheirownmediumingrowthrate(Figure-4A),andmorphology(Figure-4B)uptoandevenbeyond15populationdoublings.ThisdatashowsthatalthoughcompetitorAclaimsextendedlifespanoftheirkeratinocytesintheirownmedium,thehealthandmorphologyoftheseadultkeratinocytesispoorbeyond13-15populationdoublings,renderingtheculturesunusableforfurtherexperimentation. |
Figure4B: MorphologyofLonza’sadultkeratinocytesgrowninKGM-Gold™GrowthMediumcomparedtoCompetitorA’sadultkeratinocytesintheirmedium.
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Lonza"sAdultKeratinocytes in KGM-Gold™Medium | CompetiorA"sAdultKeratinocytes inTheirMedium | | |
Day13,15TPD | Day25,17TPD | | Findmoredatainourwhitepaper:"ASerum-freeMediumforNormalHumanEpidermalKeratinocytes(NHEK)" Registertodownload |
Day20,19TPD | Day32,19TPD | |
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ThegrowthperformanceandlongevityofCompetitorA"sadultkeratinocytesgrowninCompetitorA"sMediumwerecomparedwithLonza’sadultkeratinocytesgrowninKGM-Gold™GrowthMedium. AlthoughCompetitorAclaims≥25totalpopulationdoublingsfortheiradultkeratinocytes,actualresultsshowthatthislotbarelyobtained19PD. NotethepresenceofseveralenlargedcellsinPassage2inCompetitor’sMedium. Attheendofpassage4(15PD)Lonza’sadultkeratinocytesconsistofmostlysmallproliferativecells.
New!PurchaseourAdultKeratinocyteCellCultureKit(catalogno.192627B)andgetadiscountedpriceoncellsandmedia. Thekitconsistsofacryopreservedampuleofadultkeratinocytes(catalogno.192627)andKGM™-GoldBulletKit™GrowthMedia(catalogno.192060)