Content:20µgOverview
Stem
MACS™Lin28mRNAencodesthehumanRNA-bindingproteinLin28andisdesignedfortransienttransfection.
Applications
- Modulationofpluripotency
- Reprogramming
Details
Backgroundinformation
TheRNAbindingproteinLin28isaconservedregulatorofcellfate.Invertebrates,Lin28promotespluripotencyandcontrolsdifferentiationindiversetissues.Lin28candirectlyinhibitlet-7microRNAprocessing.Itishighlyexpressedinhumanembryonicstemcellsandhasbeenusedtoenhancethegenerationofinducedpluripotentstemcells.
Detailedprocedure
Forsatisfactorytransfectionresults,useatransfectionprotocoloptimizedforyourspecificcelltype.StemMACS™eGFPmRNAorStemMACS™NucleareGFPmRNAalloweasyevaluationoftransfectionefficiencyandarerecommendedaspositivecontrols.
Qualitydescription
StemMACS™mRNAsarehighlypure,invitro-transcribedmRNAsthathavebeencarefullyoptimizedtoensurehighlevelexpressionaftertransfection.PolyadenylationandenzymaticcapmimicafullyprocessedmaturemRNAandensureefficienttranslation.Incorporationofthemodifiednucleotidespseudouridineand5-methylcytidinereducestheinnateantiviralresponsetosingle-strandedmRNA.Robustexpressionoftheencodedfactoraftertransfectionhasbeenverifiedbyimmunofluorescenceorflowcytometry.
StemMACS™mRNAsarelyophilizedfroma0.2µMfilteredsolutionforextendedshelf-life.