Content:20µgOverview
Stem
MACS™eGFPmRNAencodesenhancedgreenfluorescentprotein(eGFP)fortransienttransfection.
Applications
- PositivecontrolformRNAtransfections
- OptimizationofmRNAtransfectionprotocols
Details
Backgroundinformation
StemMACS™eGFPmRNAwasdesignedfortransientexpressionoftheeGFPproteinaftertransfection.FluorescenceoftheeGFPproteincanbeeasilydetectedbyfluorescencemicroscopyorflowcytometry.StemMACS™eGFPmRNAisthereforeidealformonitoringandoptimizingtransfectionefficiencyandisrecommendedasapositivecontrolformRNAtransfections.
Detailedprocedure
Forsatisfactorytransfectionresults,useatransfectionprotocoloptimizedforyourspecificcelltype.StemMACS™eGFPmRNAorStemMACS™NucleareGFPmRNAalloweasyevaluationoftransfectionefficiencyandarerecommendedaspositivecontrols.
Qualitydescription
StemMACS™mRNAsarehighlypure,invitro-transcribedmRNAsthathavebeencarefullyoptimizedtoensurehighlevelexpressionaftertransfection.PolyadenylationandenzymaticcapmimicafullyprocessedmaturemRNAandensureefficienttranslation.Incorporationofthemodifiednucleotidespseudouridineand5-methylcytidinereducestheinnateantiviralresponsetosingle-strandedmRNA.Robustexpressionoftheencodedfactoraftertransfectionhasbeenverifiedbyimmunofluorescenceorflowcytometry.
StemMACS™mRNAsarelyophilizedfroma0.2µMfilteredsolutionforextendedshelf-life.