Content:20µgOverview
Stem
MACS™CreRecombinasemRNAencodesacodon-optimizedvariantofCrerecombinasefortransienttransfection.
Applications
- site-specificrecombinationbetweenloxPsites
Details
Backgroundinformation
CrerecombinaseisatyrosinerecombinaseenzymederivedfromtheP1bacteriophagethatcatalyzessite-specificrecombinationbetweentwoDNArecognitionsites(loxPsites).
Detailedprocedure
Forsatisfactorytransfectionresults,useatransfectionprotocoloptimizedforyourspecificcelltype.StemMACS™eGFPmRNAorStemMACS™NucleareGFPmRNAalloweasyevaluationoftransfectionefficiencyandarerecommendedaspositivecontrols.
Qualitydescription
StemMACS™mRNAsarehighlypure,invitro-transcribedmRNAsthathavebeencarefullyoptimizedtoensurehighlevelexpressionaftertransfection.PolyadenylationandenzymaticcapmimicafullyprocessedmaturemRNAandensureefficienttranslation.Incorporationofthemodifiednucleotidespseudouridineand5-methylcytidinereducestheinnateantiviralresponsetosingle-strandedmRNA.Robustexpressionoftheencodedfactoraftertransfectionhasbeenverifiedbyimmunofluorescenceorflowcytometry.
StemMACS™mRNAsarelyophilizedfroma0.2µMfilteredsolutionforextendedshelf-life.