Content:20µgOverview
Stem
MACS™CebpbmRNAencodesthetranscriptionfactorC/EBP-betafortransienttransfection.
Details
Backgroundinformation
CC
AAT/enhancer-bindingproteinbeta(C/EBP-beta)isamemberofthebasicleucinezipper(bZIP)containingfamilyoftranscriptionfactors.ItcanfunctionasahomodimerorinheterodimericcomplexeswithotherC/EBPfamilymembers,suchasC/EBPalpha,-betaor-gamma.C/EBP-betaplaysaroleintheregulationofgenesthatareimportantforimmuneandinflammatoryresponses.Inaddition,C/EBP-betaistransientlyinducedduringtheearlystagesof
ADIpocytedifferentiationandpromotes
AdipogenesisincooperationwithotheradipogenicfactorssuchasPPAR-gamma.InpluripotentstemcellresearchoverexpressionofC/EBP-betahasbeenusedtoinducethedifferentiationintoadiposetissue.
Detailedprocedure
Forsatisfactorytransfectionresults,useatransfectionprotocoloptimizedforyourspecificcelltype.StemMACS™eGFPmRNAorStemMACS™NucleareGFPmRNAalloweasyevaluationoftransfectionefficiencyandarerecommendedaspositivecontrols.
Qualitydescription
StemMACS™mRNAsarehighlypure,invitro-transcribedmRNAsthathavebeencarefullyoptimizedtoensurehighlevelexpressionaftertransfection.PolyadenylationandenzymaticcapmimicafullyprocessedmaturemRNAandensureefficienttranslation.Incorporationofthemodifiednucleotidespseudouridineand5-methylcytidinereducestheinnateantiviralresponsetosingle-strandedmRNA.Robustexpressionoftheencodedfactoraftertransfectionhasbeenverifiedbyimmunofluorescenceorflowcytometry.
StemMACS™mRNAsarelyophilizedfroma0.2µMfilteredsolutionforextendedshelf-life.