Content:20µgOverview
Stem
MACS™c-MycmRNAencodesast
ABIlizedversionofthetranscriptionfactorc-Mycfortransienttransfection.
Applications
- Modulationofpluripotency
- Reprogramming
Details
Backgroundinformation
c-Mycisamultifunctional,nuclearphosphoproteinthatplaysaroleincellcycleprogression,apoptosisandcellulartransformation.Itregulatestranscriptionofavarietyoftargetgenes.Togetherwithothertranscriptionfactorsc-Mychasbeenusedforthegenerationofinducedpluripotentstemcells.
Detailedprocedure
Forsatisfactorytransfectionresults,useatransfectionprotocoloptimizedforyourspecificcelltype.StemMACS™eGFPmRNAorStemMACS™NucleareGFPmRNAalloweasyevaluationoftransfectionefficiencyandarerecommendedaspositivecontrols.
Qualitydescription
StemMACS™mRNAsarehighlypure,invitro-transcribedmRNAsthathavebeencarefullyoptimizedtoensurehighlevelexpressionaftertransfection.PolyadenylationandenzymaticcapmimicafullyprocessedmaturemRNAandensureefficienttranslation.Incorporationofthemodifiednucleotidespseudouridineand5-methylcytidinereducestheinnateantiviralresponsetosingle-strandedmRNA.Robustexpressionoftheencodedfactoraftertransfectionhasbeenverifiedbyimmunofluorescenceorflowcytometry.
StemMACS™mRNAsarelyophilizedfroma0.2µMfilteredsolutionforextendedshelf-life.