Regulatorystatus:Forresearchuseandexvivocellprocessingonly
AvailABIlity:worldwide(1)
Content:0.2mgin1mLOverview
MACSGMPCD3pureisaGMP-gradesolubleCD3(OKT3)antibodyfor
exvivoTcellactivation.
Disclaimer
MACSGMPProductsareforresearchuseandexvivocellcultureprocessingonly,andarenotintendedforhumaninvivoapplications.ForregulatorystatusintheUSA,pleasecontactyourlocalrepresentative.
Details
Backgroundinformation
TheCD3antibody(OKT3)recognizesthehumanCD3antigenwhichisexpressedonmatureTcells,thymocytesandNKTcells.CD3isassociatedwiththeTcellreceptor(TCR)andinducesthesignalingcascadeforeffectorfunctionsoftheTcell.
ActivationandproliferationofTcellscanbeinducedbyinteractionoftheTcellreceptor(TCR)withpeptide-MHCcomplexesexpressedonthesurfaceofantigenpresentingcells(APC).Tcellactivationcanalsobeachievedbyago
NISTicstimulationwiththeCD3antibodybindingtotheTCRcomplex.
Applications
Theantibodyissuitablefor
exvivoTcellactivationandexpansionandisalreadyestablishedinavarietyofclinicalprotocolsfor
exvivoTcellstimulation.Forexample,thetransductionofTcellswithviralvectorsrequiresthepreviousactivation,whichcanbeachievedbystimulationwithCD3antibody
1,2.Fur
Thermore,Tcellsubsets,suchastumorinfiltratinglymphocytes(TILs)canbeexpanded
3,orTcellspolyclonallyactivatedbyCD3stimulation.
TheCD3antibodycanbecombinedwithCD28antibody,whichgivesanadditionalcostimulatorystimulus4.
Qualitystatement
MACSGMPProductsaremanufacturedandtestedunderaqualitymanagementsystem(ISO13485)andareincompliancewithrelevantGMPguidelines.Theyaredesignedfollowingtherecommendationsof
USP<1043>onancillarymaterials.