Regulatorystatus:Forresearchuseandexvivocellprocessingonly
AvailABIlity:worldwide(1)
Source:E.coli
Content:25µgOverview
Interleukin-1β(IL-1β)isapro-inflammatorycytokinethatmediatesinflammatoryresponsesanddisplaysawidevarietyof
BIOLOGicalactivitiesonmanydifferentcelltypes,includingTcells,Bcells,andmonocytes.
MACSGMPRecombinantHumanIL-1βisdesignedfor
exvivocellcultureprocessing.Noanimal-orhuman-derivedmaterialswereusedformanufactureofthisproduct.Theproductislyophilizedwithoutcarrierproteinorpreservatives.
Disclaimer
MACSGMPProductsareforresearchuseandexvivocellcultureprocessingonly,andarenotintendedforhumaninvivoapplications.ForregulatorystatusintheUSA,pleasecontactyourlocalrepresentative.
Details
Backgroundinformation
IL-1βismainlysecretedbymonocytesandmacrophages.IL-1βsecretionhasalsobeenreportedforavarietyofothercells,includingBcells,NKcells,dendriticcells,astrocytes,andmicroglialcells.IL-1βactsalsoonnonimmunesystemcellssuchasfibroblasts,vascularendothelialcells,andhepatocytes.
Applications
MACSGMPRecombinantHumanIL-1βcanbeusedforavarietyofapplications,includingtheexvivogenerationofhumandendriticcellsfromenrichedCD14+monocytepopulations.2–4
Biologicalactivity
ThespecificactivityisdeterminedbyproliferationassayaccordingtoPooleandGaines-Das
1usingD10.G4.1cellsprovidedbytheGermanResourceCentreforBiologicalMaterial(DSMZ).TheproliferationassaywascalibratedwiththefirstinternationalreferencestandardforhumanIL-1β(
NIBSCcode86/680)providedbytheNationalInstituteforBiologicalStandardsandControl.
Specificactivity:≥2×107IU/mg
Qualitystatement
MACSGMPProductsaremanufacturedandtestedunderaqualitymanagementsystem(ISO13485)andareincompliancewithrelevantGMPguidelines.Theyaredesignedfollowingtherecommendationsof
USP<1043>onancillarymaterials.