Regulatorystatus:Forresearchuseandexvivocellprocessingonly
AvailABIlity:worldwide(1)
Source:E.coli
Content:100µgOverview
Fms-relatedtyrosinekinase3ligand(Flt3-Ligand)isagrowthfactorthatexertspleiotropicandpotenteffectsonthedevelopmentofhematopoieticstemcells(HSCs)andtheimmunesystem.InsynergywithothergrowthfactorsFlt3-LigandpromotesproliferationanddevelopmentofHSCs,myeloidandlymphoid
Progenitorcells,dendriticcells,andnaturalkillercells.
MACSGMPRecombinantHumanFlt3-Ligandisdesignedfor
exvivocellcultureprocessing.Noanimal-orhuman-derivedmaterialswereusedforthemanufactureofthisproduct.Theproductislyophilizedwithoutcarrierproteinorpreservatives.
Disclaimer
MACSGMPProductsareforresearchuseandexvivocellcultureprocessingonly,andarenotintendedforhumaninvivoapplications.ForregulatorystatusintheUSA,pleasecontactyourlocalrepresentative.
Details
Backgroundinformation
Flt3-Ligandbelongstoasmallfamilyofα-helicalcytokinesandisexpressedbyTlymphocytesandbonemarrowstromalfibroblastsasamembrane-boundandasolubleisoform.BothisoformssignalthroughthetyrosinekinasereceptorFlt3/Flk-2,whichisrestrictedtocellsofhematopoieticorigin.
Applications
MACSGMPRecombinantHumanFlt3-Ligandcanbeusedforavarietyofapplications,includingtheexpansion2,3andtheviraltransduction4,5ofhematopoieticstemandprogenitorcellsderivedfrombonemarrow,peripheralbloodcells,andcordblood.
ThespecificactivityisdeterminedbyproliferationassayaccordingtoMeyerandDrexler
1usingOCI-AML5cells.TheproliferationassaywascalibratedwiththereferencestandardforhumanFlt3-Ligand(
NIBSC96/532)providedbytheNationalInstituteforBiologicalStandardsandControl.
Specificactivity:≥4×105IU/mg
Qualitystatement
MACSGMPProductsaremanufacturedandtestedunderaqualitymanagementsystem(ISO13485)andareincompliancewithrelevantGMPguidelines.Theyaredesignedfollowingtherecommendationsof
USP<1043>onancillarymaterials.