Regulatorystatus:Forresearchuseandexvivocellprocessingonly
AvailABIlity:worldwide(1)
Source:E.coli
Content:100µgOverview
Epidermalgrowthfactor(EGF)isasmallmitogenicpolypeptidethatstimulatestheproliferationanddifferentiationofawidevarietyofcellsofectodermalandmesodermalorigin,includingfibroblasts,epithelialcells,andendothelialcells.
MACSGMPRecombinantHumanEGFisdesignedfor
exvivocellcultureprocessing.Noanimal-orhuman-derivedmaterialswereusedforthemanufactureofthisproduct.Theproductislyophilizedwithoutcarrierproteinorpreservatives.
Disclaimer
MACSGMPProductsareforresearchuseandexvivocellcultureprocessingonly,andarenotintendedforhumaninvivoapplications.ForregulatorystatusintheUSA,pleasecontactyourlocalrepresentative.
Details
Backgroundinformation
EGFistheprototypeofthelargefamilyofEGF-likeproteinswithacommonstructuralmotifcomprisingthreeintramoleculardisulfidebonds.EGFisproducedbyvariouscelltypeslikemammaryglandcells,gutepithelialcells,andcellsinthenervoussystemandthekidney.ProductionofEGFisinducedbytestosteroneandinhibitedbyestrogens.Invitro,EGFstimulatestheproliferationanddifferentiationofmesenchymalcells,actsasamitogenforfibroblasts,neuralprecursors,epithelialandendothelialcells,andpromotescolonyformationofepidermalcells.
Applications
MACSGMPRecombinantHumanEGFcanbeusedasaningredientformanycellcultureapplications.EGFcansupportthemaintenanceofectodermalandmesodermal
Progenitorcells,andstimulatestheproliferationanddifferentiationofawidevarietyofcelltypesderivingbothfromectodermandmesoderm.
ThespecificactivityisdeterminedbyproliferationassayaccordingtoRobinsonandGaines-Das
1using3T3cells.TheproliferationassaywascalibratedwiththeinternationalstandardforhumanEGF(
NIBSCcode91/530)providedbytheNationalInstituteforBiologicalStandardsandControl.
Specificactivity:≥5×105IU/mg
Qualitystatement
MACSGMPProductsaremanufacturedandtestedunderaqualitymanagementsystem(ISO13485)andareincompliancewithrelevantGMPguidelines.Theyaredesignedfollowingtherecommendationsof
USP<1043>onancillarymaterials.