Content:10columnsOverview
MColumnsaredesignedformolecular
BIOLOGyandproteinbiochemistryapplicationswhichrequirethebindingoflargeramountsofmolecules.
MColumnscanbeusedwiththefollowingseparators:
- MiniMACSSeparator
- OctoMACSSeparator
Columncapacity
MColumnsareusedforcelllysatesfrom3-5×107cells.
Details
Backgroundinformation
MColumnsareespeciallydesignedformolecularbiologyandproteinbiochemistryapplicationswhichrequirethebindingoflargeramountsofmolecules.Priortouse,theMColumnareplacedinaseparator,whichinducesastrongmagneticfieldwithinthecolumn.MoleculeslabeledwiththeextremelysmallMACSMicroBeadsareretainedonthecolumn,whereasunlabeledmaterialcanbeefficientlywashedaway.Targetmoleculescanbeelutedinasmall,convenientvolume.MColumnscanbeusedtoisolatedifferentmolecules,suchasmRNA,sequencespecificnucleicacids,andproteinsfornumerousapplicationslikeimmunoprecipitation,purificationofDNAandRNAbindingproteins,oranalysisofproteininteractionsormolecularcomplexes.
MColumnsareindividuallypackedinasterileway.