Capacity:for20tests(1)Overview
ThecECEnrichmentandEnumerationKitisdesignedfortheenumerationofmaturecirculatingendothelialcells(cECs)fromperipheralblood,basedontheexpressionofCD34andCD146aswellasthelackofCD45.
Toensurereliableenumerationresults,thecECareenrichedduringtheproceduretoincreasethesensitivityoftheflowcytometricanalysis.
Thekitcontainsallreagentsfor:
- redbloodcelllysis,
- cECenrichment,
- blockingofFcreceptor–mediatednon-specificlabelingofnon-targetcells,
- enumerationofcECs,
- exclusionofdeadcells,
- isotypecontrols.
Details
Backgroundinformation
ThenumberofcECsinperipheralbloodinhealthyindividualsisverylow.Toobtainreliableenumerationresultsfortheserarecells,thesensitivityofflowcytometricanalysisneedstobeincreased.ThisisachievedbytheenrichmentofcECs,thusreducingthenumberoftotaleventsthathavetobeanalyzed.
Downstreamapplications
Overthepastdecade,severalpublicationssuggestedthatthenumberofcECsdetectedinhumanperipheralwholebloodcouldbeasignificant
Markerforvasculardamageinconjunctionwithvariousdiseases,suchas
- diabetes1,
- heartdisease2,
- systemicsclerosis3,
- orcancer4.
cECsareshedfromthedamagedvasculatureandreleasedintothebloodcirculation.Inthecourseofdisease,increasednumbersofcECscanbemonitoredintheperipheralblood.
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