本产品类同于赛默飞的PageRuler™ Prestained Protein Ladder, 10 to 180 kDa（货号26616）
Formulation： 62.5 mM Tris-H3PO4, pH7.5, 5mM EDTA, 2 % (W/V) SDS, 33 % (W/V) Glycerol, 0.02 %(V/V) proclin300.
Storage： Stable at -20°C for 36 months ; 4°C for 3 months; 25°C for 4 weeks
Quality Control：Tested in SDS-PAGE and Western Blotting.
1U Prestained Protein Ladder is a ready-to-use formulation which does not require boiling, contains 10
prestained proteins covering a wide range of molecular weights (10~180 kDa) and includes a orange reference
band at 70 kDa and a green reference band at 10 kDa. The protein ladder is designed for monitoring protein
separation during SDS-PAGE, verification of Western blot transfer (PVDF, nylon or nitrocellulose membranes) and
for the approximate sizing of proteins. Supplied as a ready-to use formulation in gel loading buffer, the ladder
requires no heating, dilution or addition of reducing agents.
Lot-to-lot variation of apparent molecular weight of prestained proteins is less than 1%.
Features & Recommended Usage :
△ Sharp and stable bands
△ Two reference bands (orange at 70 kDa; green at 10 kDa)
△ Ready-to use
△ Western Blot: 3~5 μl/Lane
1~2μl/Lane (15-Lane mini-gel)
2~3μl/Lane (10-Lane mini-gel)
Do not boiling, dilution or addition of reducing agents!!
Migration patterns of 1U Prestained Protein Ladder：
The apparent molecular weight of each protein (kDa) has been
determined by calibration against an unstained protein ladder in