CloneCRA1detectsthealphasubunitofthehumanhigh-affinityFcreceptorforIgE.Thereceptoriscomposedofonealpha,onebetaandtwodisulfid-linkedgammasubunits,ofwhichthealphasubunitbindsIgE1.FcεRIαisexpressedonmastandbasophiliccellsandisup-regulatedinthepresenceofIgE2,3.TheFcεRIcomplexplaysanimportantroleintriggeringIgE-mediatedallergicreactions.AntibodycloneCRA1doesnotinhibitbindingofIgE4.Itinducesmastcellactivation,followedbyhistaminrelease5.
Thispure-functionalgradeantibodyissuitedforcellculturefunctionalassays,forexample,blockingofreceptor-ligandbinding.