TheACSA-2antigenisspecificallyexpressedonGLAST(ACSA-1)positiveastrocytesandisthereforeanexclusive
Markerofastrocytesinthedevelopingandneonatalmousecentralnervoussystem(CNS).TheAnti-ACSA-2MicroBeadsallowalsoforpurificationofastrocytesthatshowweakGLAST(ACSA-1)expression.
ThepercentageofACSA-2positiveastrocytesdiffersaccordingtothemouseageandthebrainregionusedforthecellisolation.
TheisolationofprimaryastrocyteswastestedparticularlyondissociatedpostnatalCD-1mousebraintissuederivedfromanimalsyoungerthanpostnatalday8(P8).Theprotocolcanbeperformedinonlyonehour.Theastrocytesshowexcellentmorphologyincultureusing
MACS®NeuroMedium,MACS®NeuroBrew-21withP/SandL-glutamine.